丙型肝炎病毒核心基因免疫研究
丙型肝炎病毒;肝炎，丙型;基因，病毒;基因免疫,项目负责人,Correspondenceto,AstudyofgeneimmunizationwithrecombinantexpressionplasmidofhepatitisCviruscoreantigen,,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadings,摘要
第四军医大学唐都感染病院 陕西省西安市 710038项目负责人 周永兴，710038，陕西省西安市，第四军医大学唐都感染病院.
Correspondence to Yong-Xing Zhou, Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University,Xi＇an 710038, Shaanxi Province, China
收稿日期 1998-07-08
A study of gene immunization with recombinant expression plasmid of hepatitis C virus core antigen
Yong-Xing Zhou, Zhi-Hua Feng, Zhan-Sheng Jia, Jian-Qi Lian, Jin-Ge Li and Wen-Bo Li
Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi＇an 710038, Shaanxi Province, China
Abstract
AIM To study the efficacy and feasibility of DNA immunization with recombinant expression plasmid of hepatitis C virus core gene in prevention and treatment of HCV infection.
METHODS The recombinant plasmid containing HCV-C gene was constructed and expressed transiently by using lipofectamine in the SP2/0 cells. After appraisal and purification, these plasmid and control DNA were directly injected intramuscularly into Balb/c mice. The presence of HCV C specific antibody in the sera was checked by ELISA. Proliferative responses of spleen cells from experimental and control mice were determined by 3H thymidine incorporation, and CTL activity was measured by standard 4-h 51Cr release assays by using SP2/0 cells transfected or uninfected plasmid pcDNAHCV-C as targets. ......
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