正常原代猪肝细胞方瓶微载体静止培养
人工肝;肝细胞;细胞,培养的;微载体,*国家自然基金资助项目，No.39570212，广东省“五个一”重点课题.,项目负责人,Correspondenceto,Cultivationofporcinehypatocytesonmicrocarriersatstandstill*,Abstract,AIM,METHODS,RESULTS,CO
第一军医大学附属珠江医院普通外科 广东省广州市 510282*国家自然基金资助项目，No.39570212，广东省“五个一”重点课题.
项目负责人 陈柯，510282，第一军医大学附属珠江医院普通外科，广东省广州市
Correspondence to Ke Chen, Department of General Surgery, Zhujiang Hospital, First Military Medical College
Guangzhou 510282, Guangdong Province, China
收稿日期 1998-06-06 接收日期 1998-12-12
Cultivation of porcine hypatocytes on microcarriers at standstill*
Ke Chen, Yi Gao, Yu-Xian Pan and Ji-Zhen Yang
Department of General Surgery Zhujiang Hospital, First Military Medical College, Guangzhou 510282, Guangdong Province, China
Abstract
AIM To explore a culturing method of porcine hypatocytes on microcarriers and intend to use those culturing cells in bioartificial liver.
METHODS Hepatocytes were isolated from 1-2 months iocal pig by the two-step collagenase perfusion technique. 5×107 hepatocytes, 2g/L cytodex-3 microcarriers and 50mL DMEM including 100mL/L serum from porcine portal vein were inoculated into a 100mL flask. The morphology of culturing hepatocytes and concentrations of aibumin and urea in the supernatant were dynamically examined. ......
您现在查看是摘要页，全文长 11114 字符。