肝细胞癌mdm2基因表达及其与p53基因突变的关系
肝肿瘤;细胞癌,肝;mdm2基因;p53基因;基因表达,项目负责人,Correspondenceto,mdm2,geneexpressioninhepatocellularcarcinomaanditsrelationshiptop53genemutation,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadin
同济医科大学实验医学研究中心超微病理研究室 湖北省武汉市 430030孙宝华，男，1968-03-10生，山东省莱西市人，汉族. 1991年山东省滨州医学院医疗系毕业，1994年同济医科大学病理学硕士，1997年同济医科大学超微病理学博士，现为同济医院临床免疫研究室博士后，讲师，主要从事原发性肝癌发病机制的分子生物学研究，发表论文8篇.
项目负责人 孙宝华，430030，湖北省武汉市解放大道1095号，同济医科大学附属同济医院临床免疫研究室.
Correspondence to Bao-Hua Sun, Clinical Immunology Research Unit, Tongji Hospital of Tongji Medical University, 1095 Jiefang Avenue, Wuhan 430030, Hubei Province, China
Tel. +86·27·83638881 Ext. 3743
E-mail lcmys@tjh. tjmu. edu. cn
收稿日期 1998-10-08
mdm2 gene expression in hepatocellular carcinoma and its relationship to p53 gene mutation
Bao-Hua Sun, Zhong-Bi Wu, You-Bing Ruan and Mu-Lan Yang
Department of Ultrastructural Pathology, Research Center of Experimental Medicine, Tongji Medical University,Wuhan 430030, Hubei Province, China
Abstract
AIM To estimate the role of mdm2 gene overexpression in primary hepatocellular carcinoma (HCC) and its relationship to p53 gene mutation.
METHODS Silver staining PCR-SSCP was used to determine exon 5-8 mutation of p53 gene. In situ hybridization (ISH) was employed to detect the mdm2 gene overexpression at mRNA level. Strept avidin-biotin-enzyme complex method was used for immunohistochemistry detection. ......
您现在查看是摘要页，全文长 13351 字符。