刘勇,天津医科大学附属肿瘤医院胃肠肿瘤科 天津市 300060

    齐清会,大连医科大学附属第一医院普外科 辽宁省大连市 116011

    刘勇,男, 1975-10-06生, 天津市人, 汉族, 2004年天津医科大学博士毕业.

    项目负责人:刘勇, 300060, 天津市, 天津医科大学附属肿瘤医院胃肠肿瘤科. libra_ly@163.com

    收稿日期: 2004-12-13 接受日期: 2004-12-21

    Isolation and Culture of Rat Gastric InterstitialCells of Cajal

    Yong Liu, Qing-Hui Qi

    Yong Liu,Department of Gastrointestinal Tumor, Cancer Hospital of Tianjin Medical University, 300060, Tianjin, China

    Qing-Hui Qi, Surgery Department, the First Affiliated Hospital of Dalian Medical University, 116011, Liaoning Province, China.

    Correspondence to: Dr. Yong Liu, Department of Gastrointestinal Tumor, Cancer Hospital of Tianjin Medical University, 300060, Tianjin, China. libra_ly@163.com

    Received: 2004-12-13 Accepted: 2004-12-21

    Abstract

    AIM: In order to investigate the pacing mechanism of the interstitial cells of Cajal (ICC), the method for isolation and culture of ICC from the Wistar rat stomach was explored.

    METHODS: The gastric tissue of Wistar rat was dissected. Collagenase was used to isolate ICC. The cells were suspended in smooth muscle cell medium containing stem cell factor. The medium was changed every other day until the cells were used. The c-Kit antibody was applied to label ICC to distinguish them from smooth muscle cells. ......