Meng Hongzi，Li Riyong，Sun Shu，et al.

    Department of Pathology，Yanbian Medical College，Yanji133000.

    【Abstract】 Objective To study the regulation of rTNF，rIL-6，rIL-2， 60 Co-rays on the expression of the genes of P53，APC and c-Ha-ras by using human gastric carcinoma cell line MGC803as the model.Methods The expression levels of P53，APC and c-Ha-ras mRNA of human gastric carcinoma cell line MGC803were detected by in situ hybridization technique，and the expression level of the c-fos protein was detected through immunohistocheˉmical SP(Stretavidin/Peroxidase)method.Results It was found that human gastric carcinoma cell line MGC803can’t express P53and APC，but can express C-Ha-ras and c-fos highly.The results were shown as follows:The expression of P53was induced P<0.01;The expressions of c-Ha-ras and c-fos were inhibited P<0.01by rTˉNF;The expressions of c-fos was inhibited rIL-6P<0.01;The expressions of C-fos was inhibited rIL-2P<0.01.There were no genetic expressions in the early stage of irradiation(6h)，however，there were expressions of c-Ha-ras and c-fos in various degrees during the late stage.Conclusion There is the regulation of rTNF ......