【摘要】 目的 研究人胰岛素基因在转基因家蝇中所获得的表达产物对正常昆明小鼠血糖的影响。方法 用重组质粒pEF1α-ImINS转入家蝇基因家蝇精卵经G418筛选后，将F 2 代96h蝇蛆匀浆和杆状病毒感染家蝇幼虫后96h蝇蛆的匀浆，分别对昆明小鼠按50mg/只行腹腔注射。通过对注射前、后小鼠血糖值进行统计学处理，分析转基因产物隆血糖的生物学效应。结果 注射pEF1α-ImINS转基因家蝇蝇蛆后6h与注射前24h比较，差异有非常显著性;注射杆状病毒感染家蝇蝇蛆后6h与注射前24h比较，差异有非常显著性;注射正常家蝇蝇蛆后6h与注射前24h比较，差异有非常显著性;结论 pEF1α-ImINS转基因家蝇F 2 代96h蝇蛆、杆状病毒感染家蝇幼虫后96h的蝇蛆及正常家蝇96h蝇蛆对昆明小鼠均有明显的降血糖作用;首次发现正常家蝇蝇蛆的PBS匀浆对正常昆明小鼠具有降血糖作用。

    关键词 人胰岛素 表达产物 KM小鼠 家蝇 血糖

    Study on the expression of human insulin gene in houselfy

    Li Hua，Liu Weiquan，Wang Benxu，et al.

    Faculty of Laboratory Animal，Chinese Medical University，Shenyang110001.

    【Abstract】 Objective Study on the effect of the expression product of human insulin gene in housefly.Methods The KMwere divided into four groups randomly.Transgenic expression products of insulin in housefly magˉgot were then injected into KM mice intraperitoneally.And variation of the concentration of blood sugar was detercted at the time24h before injection ......