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Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis
http://www.100md.com 《细胞生物学杂志》2003年第3期
     1 Department of Cellular and Molecular Pharmacology and Department of Medicine and the Cardiovascular Research Institute, University of California, San Francisco, CA 94143)8}+2f', 百拇医药

    2 Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, D-79104 Freiburg, Germany)8}+2f', 百拇医药

    3 Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322)8}+2f', 百拇医药

    Abstract)8}+2f', 百拇医药

    Neutrophils exposed to chemoattractants polarize and accumulate polymerized actin at the leading edge. In neutrophil-like HL-60 cells, this asymmetry depends on a positive feedback loop in which accumulation of a membrane lipid, phosphatidylinositol (PI) 3,4,5-trisphosphate (PI[3,4,5]P3), leads to activation of Rac and/or Cdc42, and vice versa. We now report that Rac and Cdc42 play distinct roles in regulating this asymmetry. In the absence of chemoattractant, expression of constitutively active Rac stimulates accumulation at the plasma membrane of actin polymers and of GFP-tagged fluorescent probes for PI(3,4,5)P3 (the PH domain of Akt) and activated Rac (the p21-binding domain of p21-activated kinase). Dominant negative Rac inhibits chemoattractant-stimulated accumulation of actin polymers and membrane translocation of both fluorescent probes and attainment of morphologic polarity. Expression of constitutively active Cdc42 or of two different protein inhibitors of Cdc42 fails to mimic effects of the Rac mutants on actin or PI(3,4,5)P3. Instead, Cdc42 inhibitors prevent cells from maintaining a persistent leading edge and frequently induce formation of multiple, short lived leading edges containing actin polymers, PI(3,4,5)P3, and activated Rac. We conclude that Rac plays a dominant role in the PI(3,4,5)P3-dependent positive feedback loop required for forming a leading edge, whereas location and stability of the leading edge are regulated by Cdc42.

    Key Words: actin; PI 3-kinase; chemoattractant; pseudopod; positive feedback'l!, 百拇医药

    Introduction'l!, 百拇医药

    Leukocytes interpret gradients of chemoattractant to crawl toward sites of tissue injury and inflammation. Differentiated HL-60 cells, which look and behave like neutrophilic leukocytes (neutrophils), orient their polarity in response to such a gradient and migrate in the right direction . Like amoebae of Dictyostelium discoideum , both differentiated HL-60 cells and normal blood neutrophils polymerize actin in lamellae at the leading edge, or pseudopod, in a process that depends upon activity of phosphatidylinositol (PI) 3'-kinases (PI3Ks) and activation of Rho GTPases by PI 3,4,5-trisphosphate (PI[3,4,5]P3) , a lipid product of PI3Ks. PI(3,4,5)P3 accumulates preferentially in membranes at the leading edge . PI3K activity is required for attractants to activate two GTPases, Rac and Cdc42, in neutrophils . Paradoxically, however, activities of Rho GTPases are reciprocally required to support stimulation of PI(3,4,5)P3 accumulation. Indeed, asymmetric accumulation of PI(3,4,5)P3 and actin at the leading edge depends upon a positive feedback loop in which accumulation of the lipid activates Rho GTPases, whereas activated Rho GTPases and (probably) polymerized actin in turn increase accumulation of the lipid

    In fibroblasts, Rho GTPases coordinate many cellular responses, often by regulating formation of different actin assemblies. Rac triggers extension of lamellipodia containing arborized actin polymers, whereas Cdc42 induces extension of filopodia, finger-like protrusions formed by parallel arrays of actin filaments. Rho itself induces formation of stress fibers and stimulates contractility, which is mediated by activation of complexes of actin and myosin Inhibition of Rac or Cdc42 has been shown to disrupt polarity or chemotaxis in polarized epithelial cells , fibroblasts , T cells , and macrophages . Here we use bacterial toxins, dominant interfering point mutants, and fluorescent probes to explore the roles of Rac and Cdc42 in regulating PI(3,4,5)P3 accumulation, polarity, and chemotaxis in differentiated HL-60 cells. Our experiments identify distinct roles for Rac and Cdc42 in creating and maintaining neutrophil polarity.z$9';), http://www.100md.com

    Resultsz$9';), http://www.100md.com

    Effects of lethal toxin.nuq, 百拇医药

    We reported previously that Clostridium difficile toxin, which inhibits all known Rho GTPases , blocks PI(3,4,5)P3 accumulation at the plasma membrane of differentiated HL-60 cells. shows that Clostridium sordellii lethal toxin (LT) similarly prevented a neutrophil chemoattractant, formyl-methionine-leucine-phenylalanine (fMLP), from stimulating actin polymerization and formation of pseudopods , and membrane translocation of a fluorescent PI(3,4,5)P3 probe, the PH domain of Akt, tagged with GFP (PH-Akt-GFP). In addition, LT almost completely blocked fMLP-triggered migration across transwell filters . LT ADP-ribosylates Rac and Cdc42 and inhibits their activities without affecting those of Rho . In other experiments (unpublished data), a toxin that specifically inactivates Rho (C3 toxin) did not prevent PI(3,4,5)P3 accumulation. These effects do not reflect damage to the cells' intrinsic ability to accumulate PI(3,4,5)P3 as shown by the ability of the fMLP-unresponsive LT-treated cells to translocate PH-Akt-GFP to membranes in response to insulin a stimulus that activates PI(3,4,5)P3 accumulation by mechanisms that depend on tyrosine phosphorylation rather than on activation of a G protein–coupled receptor. These results confirm our previous inference that Rho GTPases are required for polarity and PI(3,4,5)P3 accumulation and indicate that Rac or Cdc42, but not Rho, mediates both these responses to fMLP. We reported previously that immunofluorescent staining with an mAb against PI(3,4,5)P3 showed a distribution identical to that of PH-Akt-GFP; this antibody shows 30-fold selectivity for PI(3,4,5)P3 versus PI(3,4)P2 .

    fig.ommttedpq@|&hy, 百拇医药

    LT inhibits polarization, actin polymerization, chemotaxis, and PI(3,4,5)P3 production. (a) F-actin localization in differentiated HL-60 cells stimulated by a uniform concentration of fMLP (100 nM) without pretreatment (HL-60, left) or after pretreatment with LT (200 ng/ml for 24 h; +LT, right). Bar, 10 µm. (b) Fluorescence microscopic images of differentiated HL-60 cells stably expressing PH-Akt-GFP. Cells pretreated with LT (unstimulated, left) were exposed to a uniform concentration (100 nM) of fMLP for 2 min (+fMLP, middle) followed by stimulation with insulin (100 µg/ml for 2 min; +insulin, right). Bar, 10 µm. Note that 31 of 40 cells (80%) showed PH-Akt-GFP translocation in response to insulin; none of these cells showed translocation in response to fMLP. (c) Migration in the transwell assay of differentiated HL-60 cells treated (dotted line) or not treated (solid line) with LT. Cell counts indicate cells that crossed the transwell membrane.pq@|&hy, 百拇医药

    We developed a transient transfection protocol that allowed us to identify distinct roles of Rac and Cdc42 in regulating polarity and PI(3,4,5)P3 accumulation, in determining subcellular distribution of activated Rac, and in directing cell migration . Dominant interfering mutant proteins and fluorescent probes were transiently expressed in differentiated HL-60 cells, with transfection efficiencies that varied from 10 to 20%. In populations cotransfected with two constructs, >80% of cells expressing one also expressed the other (for details see Materials and methods). Although they migrate slightly less rapidly (see Materials and methods) than do cells stably expressing PH-Akt-GFP , cells transiently expressing the lipid probe showed unchanged responses to fMLP, including morphologic polarity, formation of actin polymers, directed motility, and asymmetric accumulation of the probe for activated Rac ( 5 a, and 6 a).

    fig.ommttedzn]9a, http://www.100md.com

    Rac but not Cdc42 stimulates accumulation of PI(3,4,5)P3 and actin polymers. Spatial localization of PH-Akt-GFP (top images) or polymerized actin (middle images) in transfected differentiated HL-60 cells; bottom images show overlay of PH-Akt-GFP (green) and actin polymerization (red). Cells were transiently transfected to express either PH-Akt-GFP alone (control) or in combination with RacN17, RacV12, Cdc42N17, WASpC, or Cdc42V12 as indicated. Cells were unstimulated or stimulated for 3 min with a uniform concentration of fMLP (100 nM) and fixed. Bar, 10 µm. Note that the Cdc42V12 construct was normally functional despite its failure to induce actin assemblies in HL-60 cells. Indeed, transient expression of this Cdc42 mutant in COS-7 cells was associated with constitutive Cdc42 activity (result not shown) as assessed in a PAK-PBD pull-down assayzn]9a, http://www.100md.com

    fig.ommttedzn]9a, http://www.100md.com

    PAK-PBD-YFP reflects localization of activated Rac at the plasma membrane but not that of activated Cdc42. Localization of PAK-PBD-YFP in cells exposed for 3 min to 100 nM fMLP (a–c); in addition to PAK-PBD-YFP, the cell in b coexpressed RacN17 and the cell in c coexpressed Cdc42N17. Cells in d–f and g–i expressed constitutively active Rac or Cdc42, respectively; after 3 min exposure to fMLP, these cells were fixed and imaged for PAK-PBD-YFP fluorescence or immunofluorescence of the myc-tagged Rho GTPase as indicated. Bar, 10 µm.

    fig.ommtted/}w+-49, http://www.100md.com

    Actin polymerization is necessary for Rac-dependent PI(3,4,5)P3 accumulation. (a) Spatial distribution of PH-Akt-GFP (top) or PAK-PBD-YFP (bottom) in cells expressing RacV12 before and after exposure to latrunculin B (20 µg/ml) for the indicated times. (b) PAK-PBD-YFP and myc-tagged RacV12 show persistent colocalization at the membrane even after treatment with latrunculin B for 10 min. Bars, 10 µm./}w+-49, http://www.100md.com

    fig.ommtted/}w+-49, http://www.100md.com

    Inhibition of Cdc42 activity prevents consolidation of a single leading edge upon stimulation by a uniform concentration of chemoattractant. (a and b) Migration of differentiated HL-60 cells in a uniform concentration (100 nM) of fMLP. Cells expressed PH-Akt-GFP alone (a) or in combination with Cdc42N17 (b). (c) Outlines of cells migrating in a uniform concentration (100 nM) of fMLP. Each set of outlines represents a cell observed at 1-min intervals (denoted by colors as indicated), from 2 to 5 min after exposure to fMLP. Small circles in each outline represent the center of a PH-Akt-GFP–containing lamella at the cell periphery. The first control cell (left) and the first Cdc42N17-expressing cell (middle) are the same cells as those depicted in a and b, respectively. In a and b, the top images show the morphology of a single cell by Nomarski microscopy at the indicated times after chemoattractant stimulation; the bottom images show spatial localization of PH-Akt-GFP in the same cell at the same time points. Arrows identify leading edges. Bars, 10 µm. The cells in a and b with the outlines are shown in videos 1 and 2, respectively, available at

    fig.ommtted/%m1, http://www.100md.com

    Inhibition of Cdc42 activity blocks migration toward a point source of chemoattractant. (a–c) Migration of cells toward a point source of fMLP (10 µM fMLP in a micropipette). Cells expressed PH-Akt-GFP alone (a) or in combination with Cdc42N17 (b) or WASpC (c). The top images show the morphology of a single cell by Nomarski microscopy at the indicated times after chemoattractant stimulation; the bottom images show spatial localization of PH-Akt-GFP in the same cell at the same time points. Arrows identify leading edges. Bars, 10 µm. The experiments in a and b are shown in videos 3 and 4, respectively, available at . (d) Outlines of migrating cells observed after exposure to an fMLP-containing micropipette (locations indicated by asterisks). As in c, each set of outlines represents a cell observed at 1-min intervals (denoted by colors as indicated), from 0 to 3 min after exposure to the micropipette. Small circles in each outline represent the center of a PH-Akt-GFP–containing lamella at the cell periphery./%m1, http://www.100md.com

    Role of Rac as a mediator of PH-Akt-GFP translocation and polarity

    We assessed effects of Rho GTPase mutants on morphology, actin accumulation, and PH-Akt-GFP translocation at 1 or 3 min after adding fMLP ( respectively). In 17 of 19 control cells (90%) at the earlier time point (; photomicrographs in Fig. S1, available at ), fMLP induced robust recruitment of PH-Akt-GFP to the plasma membrane. By 3 min, control cells showed asymmetric recruitment of PH-Akt-GFP to the leading edge where it colocalized with polymerized actin.5, 百拇医药

    fig.ommtted5, 百拇医药

    Rac but not Cdc42 participates in PI(3,4,5)P3 accumulation5, 百拇医药

    Similarly, fMLP caused localization to the pseudopod of a fluorescent probe that detects activated Rac , PAK-PBD-YFP, the YFP-tagged p21-binding domain (PBD) of p21-activated kinase (PAK). Although in vitro this PBD binds the activated, GTP-bound forms of both Rac and Cdc42 , its YFP-tagged counterpart (PAK-PBD-YFP) predominantly detects Rac-GTP rather than Cdc42-GTP as shown by other data in . Overexpression of the constitutively active Rac mutant, RacV12, caused PAK-PBD-YFP to localize in lamellae surrounding the circumference of flat, spread-out cells in a distribution similar to that of actin and PH-Akt-GFP in RacV12-expressing cells . Moreover, a myc epitope attached to RacV12 virtually superimposed on PAK-PBD-YFP fluorescence, suggesting that the fluorescent probe faithfully detects the GTP-bound form of Rac . In contrast, the constitutively active Cdc42 mutant, CdcV12, caused no localization of PAK-PBD-YFP to the cell periphery (, g–h); instead, PAK-PBD-YFP appeared to localize with myc-tagged Cdc42V12 in a punctate cytoplasmic distribution. Although PAK-PBD-YFP might be expected to sequester GTP-bound forms of Rac, Cdc42, or both, its expression did not detectably alter neutrophil responses to fMLP. This probably reflects relatively higher concentrations of Rac-GTP versus PAK-PBD-YFP in activated HL-60 cells. Indeed, inhibition of ruffling in fibroblasts was seen only with high concentrations of PAK-PBD-YFP , making it possible to use lower concentrations in studying Rac-PAK-PBD-YFP with fluorescence resonance energy transfer.

    Rac stimulates actin polymerization by several mechanisms in different cell types . Chemoattractant-induced actin polymerization is impaired in neutrophils of mice lacking Rac2 or of a patient carrying a dominant inhibitory Rac2 mutation . In accord with these observations, the effects of overexpressed Rac mutantsindicated that Rac plays an essential role in stimulating actin polymerization, pseudopod formation, motility, and PI(3,4,5)P3 accumulation in differentiated HL-60 cells.e[*f%$c, 百拇医药

    The inhibitory effect of RacN17 on activity of endogenous Rac is thought to result from sequestering the guanine nucleotide exchange factors (GEFs) that activate the endogenous Rho GTPase . Resting (untreated) RacN17-expressing cells were flat and spread-out , unlike resting control cells. Further, RacN17 blocked PH-Akt-GFP translocation responses to fMLP and markedly reduced accumulation of polymerized actin ( see also Fig. S1). After exposure to fMLP for 3 min, HL-60 cells failed to polarize or to accumulate PAK-PBD-YFP in pseudopods or even at the cell periphery ( versus a). However, failure to translocate PH-Akt-GFP to membranes did not indicate inability to synthesize PI(3,4,5)P3 as shown by the fact that insulin stimulated PH-Akt-GFP accumulation equally well in control and RacN17-expressing cells (83 versus 81%; and Fig. S1). A minority (33%) of RacN17-expressing cells did translocate PH-Akt-GFP in response to fMLP . We were unable to quantitate RacN17 expression in individual cells to test the possibility that this mutant was expressed less strongly in cells that showed PH-Akt-GFP responses to fMLP. Presence or absence of the actin response to fMLP did track reliably with presence or absence, respectively, of PH-Akt-GFP translocation.

    As expected, inhibition of polarity and actin polymerization by RacN17 was accompanied by severely impaired motility, both in a uniform concentration of chemoattractant and in response to a gradient of fMLP supplied by micropipette (Fig. S2, a and c, available at ). Again as expected, expression of constitutively active Rac (RacV12) caused formation of actin-containing ruffles and translocation of PH-Akt-GFP to the cell periphery in 72% of cells expressing the mutant . Note that RacV12, unlike fMLP, did not induce cell polarity or migration; instead, actin and PI(3,4,5)P3 appear to have been distributed more or less uniformly about the cell periphery.[s[c4qn, 百拇医药

    When exposed to fMLP, many RacV12-expressing cells showed a paradoxical response: disappearance of ruffles and translocation of membrane PH-Akt-GFP back to the cytoplasm (unpublished data). Overexpression of activated Rac for 24 h appears not simply to have prevented fMLP from acting but instead has converted a positive response to a negative one. A satisfying explanation for this paradox awaits results of future experiments (see Discussion).

    3 translocation requires actin polymers">RacV12-induced PI(3,4,5)P3 translocation requires actin polymersxt&1j, 百拇医药

    Although PI(3,4,5)P3 is required for fMLP-stimulated Rac activation in neutrophils , effects of the Rac mutants in HL-60 cells indicate that the converse is also true: active Rac is also both sufficient and necessary to stimulate accumulation of PI(3,4,5)P3 . We have proposed that reciprocal activation of one another by Rho GTPases and PI(3,4,5)P3 constitutes a positive feedback mechanism that amplifies polarity and formation of actin polymers in response to fMLP. Such a feedback loop should be susceptible to activation at multiple points within the loop as shown by the ability of exogenous PI(3,4,5)P3 to stimulate PI3K- and Rho GTPase-dependent actin polymerization, polarity, and motility in HL-60 cells . It is likely that RacV12 mimics one arc of this loop to stimulate PI(3,4,5)P3 accumulation.

    We suggested previously that actin plays an important role in Rac-stimulated PI(3,4,5)P3 accumulation based on effects of latrunculin B, a protein that sequesters monomeric actin and stops de novo formation of actin polymers . Exposure of latrunculin-treated cells to a uniform concentration of fMLP did stimulate translocation of PH-Akt-GFP to the plasma membrane, but the translocation was short lived and did not go on to polarize in an asymmetric distribution, unlike that seen in cells capable of polymerizing actin. By inference, formation of actin polymers is necessary for sustained accumulation of PI(3,4,5)P3 in response to fMLP.71z@f, 百拇医药

    To ask whether Rac-stimulated PI(3,4,5)P3 accumulation does depend on actin polymerization, we tested the effect of exposing RacV12-expressing cells to latrunculin in the absence of fMLP stimulation. Latrunculin caused PH-Akt-GFP to translocate back from plasma membrane to cytosol . This effect was reproducible: 72% of RacV12-expressing control cells examined showed PH-Akt-GFP located at the plasma membrane, whereas after treatment with latrunculin only 16% of RacV12-expressing cells did so . In contrast, latrunculin did not cause PAK-PBD-YFP, the probe for activated Rac, to return to the cytoplasm . Instead, PAK-PBD-YFP remained at the membrane where it colocalized with myc-tagged RacV12 . We infer that, at least in the absence of fMLP, stimulation of membrane PI(3,4,5)P3 accumulation by Rac is mediated by (or depends on) actin polymerization.

    Cdc42 controls the number and stability of pseudopods&jttsx0, http://www.100md.com

    Results in indicate that Cdc42 can control the stability, number, and directionality of pseudopods but in contrast to Rac is neither necessary nor sufficient for actin polymerization or PI(3,4,5)P3 accumulation. Effects of inhibiting endogenous Cdc42 with the Cdc42N17 mutant, described below, were unexpected. For this reason, we tested a second Cdc42 inhibitor, WASpC, which is thought to act by a different mechanism, that is, by sequestering Cdc42-GTP rather than by titrating and inhibiting Cdc42 GEFs. WASpC, a fragment of Wiskott-Aldrich Syndrome protein (WASp), binds the GTP-bound form of Cdc42 but lacks COOH-terminal sequences necessary for interacting with downstream components responsible for Cdc42-dependent polymerization of actin . Effects of WASpC and Cdc42N17 proved indistinguishable, making it much more likely that the functional phenotypes were caused by reduced amount or activity of Cdc42-GTP. Unlike the effect of RacN17, inhibition of Cdc42 failed to block three characteristic responses to a uniform concentration of fMLP: membrane translocation of PH-Akt-GFP , accumulation of F-actin in lamellae , and accumulation of PAK-PBD-YFP in lamellae (.

    However, inhibiting Cdc42 did profoundly change the polarized morphology of cells observed 3 min after exposure to fMLP . In contrast to the relatively stereotyped polar morphology of control cells treated with fMLP, cells expressing Cdc42N17 or WASpC often showed multiple lamellae containing F-actin and PH-Akt-GFP; some exhibited a single pseudopod, which was usually wider and less well defined that of control cells. (For photomicrographs of actin and PH-Akt-GFP distribution in six Cdc42N17 expressing cells, see Fig. S3, available at ; Fig. S2 b shows a second WASp{Delta} C-expressing cell.)65osvm, 百拇医药

    Videomicroscopy revealed that the diverse morphologies of Cdc42-inhibited cells treated with fMLP reflected marked instability of their pseudopods (; videos 1 and 2, available at ). a shows the response of a typical control cell to uniform fMLP: the cell polarized, formed a single pseudopod, and crawled efficiently in one direction for several minutes. b shows the quite different response of a Cdc42N17-expressing cell to uniform fMLP: this cell formed a somewhat broad pseudopod, which then split into two distinct lamellae, followed by formation of a lamella at its opposite edge. Consequently, the cell migrated only a short distance compared with the control. c summarizes the migration and shape changes of randomly picked control and Cdc42-inhibited cells over a 3-min period (2–5 min after exposure to uniform fMLP). Unlike the four control cells, Cdc42-inhibited cells formed unstable and occasionally multiple pseudopods and migrated in an inefficient, vacillating manner (net movement of <2–3 µm over the 3-min period of observation).

    It appeared possible that a gradient of the attractant would produce normal (or nearly normal) polarity and migration in a Cdc42-inhibited cell. As reported previously , a control cell formed a single well-defined pseudopod and crawled straight toward the fMLP-containing micropipette ( video 3, available at ). (The micropipette produces an fMLP gradient that decreases by 15% over a distance of 10 µm ) In contrast, the Cdc42N17-expressing cell in first displayed two leading edges on the up-gradient side, then retracted both, and finally protruded a new leading edge in a direction away from the gradient (see also video 4, available at ). The WASpC-expressing cell shown in c exhibited a milder chemotaxis defect: this cell translocated PH-Akt-GFP into a pseudopod that first extended toward the micropipette and then retracted; net migration over 5 min was 5 µm (versus 20–25 µm for controls). The results in , span the spectrum of severity of the Cdc42-inhibited phenotype in micropipette experiments but do not reflect differences between the two inhibitory constructs. Cell outlines over 3 min of exposure to micropipette gradients show direct pointing and migration of control cells, whereas Cdc42N17- and WASpC-expressing cells pointed in multiple directions and failed to migrate toward the source of attractant . In every cell examined, inhibition of Cdc42 induced confused interpretation of the gradient, substantially reducing net movement toward the micropipette in comparison to control cells.

    The constitutively active Cdc42 mutant (Cdc42V12), unlike the corresponding RacV12 mutant, failed completely to stimulate formation of actin polymers or translocation of PH-Akt-GFP or to promote localization of the target probe, PAK-PBD-YFP, to the cell periphery (, d–f and g–i, for RacV12 and Cdc42V12, respectively). Moreover, RacV12 largely colocalized with PAK-PBD-YFP at the plasma membrane but Cdc42V12 did not . In addition, the Cdc42V12 mutant did not trigger formation of actin-containing filopodia like those it induces in fibroblasts and macrophages12x*, 百拇医药

    The reason for failure of this mutant to induce any responses in HL-60 cells is unknown, but the Cdc42V12 construct was functional (see the legend to ), and it did exert an effect on the cells in which it was expressed: transfection with myc- or GFP-tagged Cdc42V12 produced robust immunofluorescent or GFP fluorescent signals (unpublished data), respectively, and Cdc42V12-expressing cells failed to show any morphologic response or PH-Akt-GFP translocation in response to fMLP (unpublished data). Like the puzzling effect of RacV12 on fMLP responses, this result does not suggest a straightforward explanation and merits further investigation.

    Discussiont{i+ivo, 百拇医药

    Role of Rac in the positive feedback loop at the leading edget{i+ivo, 百拇医药

    We have presented evidence that Rac activity, but not that of Cdc42, is necessary and sufficient for chemoattractant-stimulated accumulation of PI(3,4,5)P3 and actin polymers in HL-60 cells. We can now assign to a single Rho GTPase, Rac, but not Rho or Cdc42, a dominant role in the positive feedback loop that amplifies polarized asymmetry of PI(3,4,5)P3 and actin polymers at the leading edge. We inferred previously existence of this PI(3,4,5)P3- and Rho GTPase-dependent positive feedback loop from several observations. First, exogenous PI(3,4,5)P3 induced PI3K-dependent polarity and migration of human neutrophils . In HL-60 cells, exogenous PI(3,4,5)P3 induced, in addition to polarity and migration, accumulation of actin polymers and translocation of PH-Akt-GFP at the leading edge . These effects were blocked not only by pharmacologic inhibition of PI3Ks but also by a toxin (C. difficile toxin B) that inhibits Rho GTPases.

    More complete understanding of the detailed molecular mechanisms underlying the feedback loop awaits future experiments. One arc of the loop, in which PI(3,4,5)P3 activates Rac, probably involves one or more PI(3,4,5)P3-dependent GEFs found in leukocytes, such as pRex-1 , Vav , or SWAP-70 . Molecular mechanisms mediating the reciprocal arc in which Rac-GTP promotes accumulation of PI(3,4,5)P3 are even less well defined. The fact that latrunculin-promoted disappearance of F-actin in RacV12-expressing cells causes PH-Akt-GFP to translocate from membranes back into the cytoplasm strongly implicates actin polymers at the leading edge as direct or indirect mediators of this arc of the feedback loop. In this arc of the loop, F-actin can clearly act downstream of Rac-GTP and upstream of PI(3,4,5)P3 accumulation, as shown by the ability of latrunculin to induce PH-Akt-GFP to translocate back into the cytoplasm of RacV12-expressing cells, whereas localization of RacV12 and the probe for activated Rac remain at the plasma membrane . How polymerized actin promotes PI(3,4,5)P3 accumulation, however, is not so clear: it may promote formation of scaffolds that increase PI(3,4,5)P3 synthesis or decrease its degradation, for instance, by recruiting or facilitating activation of one or more PI3Ks (e.g., PI3K by Gß or other PI3Ks via their p85 adaptor subunit ), by preventing access to PI(3,4,5)P3 of an inactivating phosphatase, such as PTEN , or (as in platelets ) by promoting activation of PI-4P-5-kinase, thereby increasing the concentration of the PI3K substrate, PI 4,5-bisphosphate. Although a role for actin in promoting PI(3,4,5)P3 accumulation has not been established in Dictyostelium, membrane accumulation of PI(3,4,5)P3 in this organism is regulated by changes in both synthesis and degradation of the phospholipid . For this reason we suspect that both processes will be found to participate in the actin-to-PI(3,4,5)P3 arm of the positive feedback loop in neutrophils.

    Although Rac serves as the central Rho GTPase in the PI(3,4,5)P3-dependent positive feedback loop that organizes the leading edge, this loop by itself cannot suffice to explain polarity or directional migration. Two sets of observations make this clear. First, expression of RacV12 does not trigger morphologic polarity but instead produces a cell surrounded by actin-containing ruffles and PI(3,4,5)P3 . Second, inhibiting Cdc42 profoundly destabilizes pseudopods and disrupts directional migration but does not appear to interfere with the feedback loop because fMLP can still stimulate formation of discrete pseudopods containing PI(3,4,5)P3 and Rac-GTP indicated, respectively, by localization of PH-Akt-GFP and PAK-PBD-YFP .rl.{y^3, http://www.100md.com

    RacV12 does not induce polarityrl.{y^3, http://www.100md.com

    RacV12 disrupts polarity in macrophages and in HL-60 cells fails to mimic the polarity- and motility-inducing effects of uniform extracellular concentrations of fMLP or exogenous PI(3,4,5)P3 . The latter two stimuli can promote polarized asymmetry of newly formed actin polymers and PI(3,4,5)P3 even when the stimuli themselves are presented to the cell symmetrically. In contrast, symmetrical distribution of activated Rac (that is, of RacV12) does not promote polarized asymmetric responses. From this discrepancy we may infer that both fMLP and exogenous PI(3,4,5)P3 trigger the polarity-inducing mechanism by mechanisms upstream of or in addition to Rac activation. If so, localized Rac activity (as revealed by the distribution of PAK-PBD-YFP in a) constitutes a "read-out" of the polarity mechanism, its location presumably controlled either by localized activation of appropriate Rac GEFs or by localized prevention of Rac inactivation.

    In keeping with this idea, exposure of neutrophils to a uniform concentration of attractant first induces formation of ruffles around the entire cell periphery followed a minute or so later by development of clear morphologic polarity with ruffles confined to a single pseudopod . HL-60 cells respond similarly to either uniform fMLP or to exogenous PI(3,4,5)P3 . In each case, ruffles and membrane PI(3,4,5)P3 appear first in a symmetrical distribution and later concentrate at one side of the cell. Thus, it seems likely that fMLP or exogenous PI(3,4,5)P3 do not simply induce Rac activation but instead also set in motion signals that limit spatial distribution of Rac activity. Indeed, neutrophil polarity may resemble the self-organizing polarity of the actin–myosin cytoskeleton observed in fish keratoplasts perturbed by a mechanical stimulus . Signals responsible for self-organizing polarity are not well defined but probably include contraction of actin–myosin complexes at the rear of keratoplasts , Dictyostelium amoebae , and neutrophils . In HL-60 cells, contraction of actin–myosin complexes could limit formation of arborized actin polymers, thereby restricting lamellae to one side of the cell. Such a process could amplify and consolidate PI(3,4,5)P3 accumulation at the leading edge because de novo formation of actin polymers promotes and may even be necessary for PI(3,4,5)P3 accumulation

    We speculate that a similar inhibition of actin polymerization and PI(3,4,5)P3 accumulation underlies the paradoxical ability of overexpressed RacV12 to block responses to fMLP (see Results). We propose the following speculative scenario: an activated Rac that does not depend on activation by GEFs subverts the normal Rac-dependent positive feedback mechanism, making it impossible (perhaps by soaking up limiting components) for the attractant to exert its normal effect on PH-Akt-GFP accumulation and actin polymerization; at the same time, fMLP exerts a direct negative effect on both actin polymerization and PI(3,4,5)P3 accumulation by stimulating activity of a Rho GTPase (Rho rather than Cdc42 or Rac) that promotes formation of actin–myosin complexes, which in turn reduce PI(3,4,5)P3 responsiveness to fMLP. In support of the latter part of this scenario, our unpublished experiments indicate that: (a) the pathway for fMLP-triggered Rho activation diverges quite early from fMLP-triggered Rac activation, actin polymerization, and PI(3,4,5)P3 accumulation (pertussis toxin inhibits the latter pathway but not the former) and (b) Rho-dependent signals can effectively turn off fMLP-dependent PI(3,4,5)P3 accumulation and polymerization of actin. Thus, it seems reasonable to propose that Rho activated by fMLP might strongly inhibit a Rac-dependent response that is intrinsically unable to amplify itself (that is, RacV12, as opposed to the Rac-GTP that accumulates in response to fMLP in control cells).

    Cdc42 stabilizes polarity$, 百拇医药

    Cdc42 does not participate directly in the PI(3,4,5)P3 feedback loop as indicated by the failure of Cdc42N17 to inhibit accumulation of F-actin and PI(3,4,5)P3 or localization of PAK-PBD-YFP. However, Cdc42 does appear to constrain where PI(3,4,5)P3, Rac-GTP, and F-actin accumulate and thereby consolidate formation of a single stable pseudopod. Inhibiting Cdc42 severely impairs stable polarity and motility: pseudopods appear, disappear, or move from one position on the cell surface to another within minutes, and cells migrate poorly or not at all ( see also videos 2 and 4).$, 百拇医药

    Previous studies have shown that Cdc42 inhibitors impair polarity and/or chemotaxis in several cell types, including polarized epithelial cells , T lymphocytes , fibroblasts , and macrophages . In these cells, the ability to polymerize actin was not affected, but localization of PI(3,4,5)P3 and activated Rac were not examined. The time scales (hours) of all these experiments differed remarkably from the minute-to-minute behavior observed in HL-60 cells. In a wound-healing assay, Cdc42-inhibited fibroblasts showed loss of polarity and formed lamellae around much of the cell circumference , but the cells moved very slowly at a rate of 1 cell diameter per h. In macrophages , expression of Cdc42N17 induced formation of broad leading lamellae and impaired interpretation of the chemoattractant gradient as in HL-60 cells. In contrast to HL-60 , such macrophages migrated faster than control cells , but neither type of macrophage moved more than 1.5–2 cell diameters in 3 h. Thus, our experiments show for the first time that Cdc42 can control polarity and migration by regulating cellular events that take place in minutes. These results make it unlikely, for instance, that Cdc42 controls polarity primarily by events that require synthesis of new proteins (as is certainly the case in yeast cells ).

    We can only speculate about the mechanisms by which Cdc42 stabilizes the pseudopod in HL-60 cells and promotes oriented polarity in other eukaryotes. Cdc42 could limit actin polymerization and accumulation of PI(3,4,5)P3 at the lateral edges of a pseudopod, perhaps by regulating formation of scaffolded complexes that inhibit Rac or promote activation of Rho at these locations. Alternatively, Cdc42 could regulate localization of pseudopods indirectly by controlling vesicle traffic and membrane localization of (for instance) enzymes that synthesize or degrade PI(3,4,5)P3. In yeast, Cdc42 stabilizes the location of a bud site, and localized Cdc42 activity polarizes the actin cytoskeleton and secretion of newly synthesized proteinss({!!, http://www.100md.com

    Materials and methodss({!!, http://www.100md.com

    Materialss({!!, http://www.100md.com

    LT was prepared as described . Human fibronectin was from . Latrunculin B was from . Human albumin (low endotoxin), DMSO, fMLP, and insulin were from . The mouse monoclonal anti-PI(3,4,5)P3 antibody was from Echelon. Mouse mAb against the 9E10 (Myc) epitope tag was purchased from Roche, Texas red–conjugated goat anti–mouse was from and rhodamine- and Alexa 647–conjugated phalloidin were from Molecular Probes. Transwell plates were from Corning. Femtotips were from Eppendorf. Electroporation cuvettes were obtained from Labrepco. The Electroporation chamber (model series 1600) was from GIBCO-BRL.

    DNA constructs2h/c|g!, 百拇医药

    Myc-tagged V12 and N17 mutants of Rac and Cdc42 were cloned in pTET7 downstream of a tetracycline repressor binding sequence as described . To activate expression of the mutant proteins, these constructs were cotransfected with a plasmid constitutively expressing a tetracycline repressor VP16 fusion protein. WASpC was as described . PAK-PBD-YFP was a gift from Klaus Hahn (Scripps Institute of Immunology, La Jolla, CA); encoded in a pcDNA3 vector, this construct comprised residues 65–150 of human PAK1 with EYFP linked to its COOH terminus by a four-residue linker (Leu-Ser-Gly-Arg).2h/c|g!, 百拇医药

    Cell culture, transfection, and toxins2h/c|g!, 百拇医药

    Procedures for cultivation and differentiation of HL-60 and HL-60 cells stably expressing PH-Akt-GFP have been described. For transient transfections, differentiated HL-60 cells (on day 6 after addition of DMSO) were washed once in RPMI-HEPES and resuspended in the same medium to a final concentration of 108 ml-1. DNA was then added to the cells (30 µg of PH-Akt-GFP or PAK-PBD-YFP DNA plus 50 µg of other constructs to be cotransfected), the cell DNA mixture was incubated for 10 min at RT, transferred to electroporation cuvettes, and subjected to an electroporation pulse on ice at 310 V, 1180 µF and low resistance. Transfected cells were allowed to recover for 10 min at RT and then transferred to 20 ml complete medium. Subsequent assays were performed 16–24 h after transfection. Although cells transiently transfected with GFP or PH-Akt-GFP migrated more slowly in response to fMLP than stably transfected cells (5 versus 7 µm per min; unpublished data), their abilities to polarize and move toward a micropipette source were unaffected.

    Differentiated HL-60 cells cultured in differentiation medium were pretreated with LT (200 ng/ml) for 24 h at 37°C. For the latrunculin experiment, cells transiently expressing RacV12 and PH-Akt-GFP were allowed to adhere to fibronectin-coated coverslips and were then treated with 20 µg/ml latrunculin B as describedr|(a, 百拇医药

    Pull-down assay for activated Cdc42r|(a, 百拇医药

    COS-7 cells were transfected with Cdc42N17 or empty vector using the Lipofectamine-Plus. The pull-down assay for active Cdc42 was based upon a previously reported procedure . After 24 h incubation in DME-H21 with 0.2% FBS, cells were trypsinized and washed with serum-free medium and resuspended at 8 x 106 ml-1 in HBSS medium with FISH buffer containing protease inhibitors. Cells were lysed, and the lysate were incubated for 45 min with glutathione-sepharose beads linked to PAK-PBD-GST. Immunoblots of the bead fractions were blotted with anti-Cdc42 rabbit polyclonal antibody and anti–rabbit IgG/HRP

    Assays and microscopic analysis6k, 百拇医药

    Transwell assays were performed as described . For spatial localization of fluorescent probes (PH-Akt-GFP or PAK-PBD-YFP) and actin in fixed cells, cells were subjected to one of the following stimulation conditions as described: no stimulation (basal), stimulation with fMLP (100 nM) for 1 or 3 min, or insulin (100 µg ml-1) for 3 min. Cells were then fixed and immunostained for the myc epitope tag fused to the NH2 terminus of mutant Rac and Cdc42. The myc antibody was used at a dilution of 1:1,000, and immunostaining was conducted as described previously . F-actin was assessed by incubating for 15 min with 10 U ml-1 rhodamine-phalloidin or Alexa 647–phalloidin as described . For all experiments in fixed cells, except those involving insulin stimulation, expression of mutant proteins was confirmed by immunostaining. In the case of insulin stimulation, even in control cells the weak PH-Akt-GFP recruitment was lost upon permeabilizing cells during the immunostaining protocol.

    Live cells were imaged after stimulation either with a uniform concentration of chemoattractant (fMLP, 100 nM) or by a point source of chemoattractant from a micropipette containing 10 µM fMLP (generated by Femtotips) as described . Since >80% of the cells expressed both constructs, fluorescence associated with PH-Akt-GFP was used to infer coexpression of the mutant protein in living cells. Time-lapse videomicroscopy was performed as described .7, http://www.100md.com

    Online supplemental material7, http://www.100md.com

    The time-lapse experiments shown in and are also viewable as Quick-Time videos available at . Videos 1 and 2 show the responses of transiently transfected differentiated HL60 cells expressing either PH-Akt-GFP alone or in combination with Cdc42N17 to a uniform concentration of chemoattractant. Videos 3 and 4 show cells expressing the same constructs responding to a point source of chemoattractant from a micropipette. Fig. S1 shows the early membrane recruitment of PH-Akt-GFP in transfected HL60 cells different mutant Rho GTPases upon stimulation by a uniform concentration of fMLP or insulin. Fig. S2 shows the migration of differentiated HL60 cells expressing RacN17 or WASpC in response to a uniform concentration of fMLP or to fMLP supplied by micropipette. Fig. S3 shows the range of phenotypes observed upon inhibition of Cdc42 activity.

    Acknowledgments}g?, http://www.100md.com

    We thank Paul Herzmark for technical assistance with microscopy. Members of the Bourne laboratory and colleagues (Diane Barber, Shaun Coughlin, Wendell Lim, Jack Taunton, Orion Weiner, and Arthur Weiss) offered valuable advice and comments on the article.}g?, http://www.100md.com

    This work was supported by National Institutes of Health grant GM-27800 (to H.R. Bourne), a grant from the Leukemia and Lymphoma Society (to S. Srinivasan), postdoctoral fellowships from the American Heart Association (to S. Glavas), the Deutsche Forschungsgemeinschaft (to A. Ott), and National Institutes of Health training grant HL07713 (to F. Wang).}g?, http://www.100md.com

    Submitted: 30 August 2002}g?, http://www.100md.com

    Revised: 18 December 2002}g?, http://www.100md.com

    Accepted: 18 December 2002}g?, http://www.100md.com

    References}g?, http://www.100md.com

    Allen, W.E., D. Zicha, A.J. Ridley, and G.E. Jones. 1998. A role for Cdc42 in macrophage chemotaxis. J. Cell Biol. 141:1147–1157.

    Ambruso, D.R., C. Knall, A.N. Abell, J. Panepinto, A. Kurkchubasche, G. Thurman, C. Gonzalez-Aller, A. Hiester, M. deBoer, R.J. Harbeck, et al. 2000. Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation. Proc. Natl. Acad. Sci. USA. 97:4654–4659.k{\:, 百拇医药

    Baldacini, O., G.A. Green, R. Girardot, B. Rihn, and H. Monteil. 1990. Fast purification of Clostridium sordellii cytotoxin. J. Chromatogr. 528:357–369.k{\:, 百拇医药

    Benard, V., B.P. Bohl, and G.M. Bokoch. 1999. Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases. J. Biol. Chem. 274:13198–13204.k{\:, 百拇医药

    Cassimeris, L., H. McNeill, and S.H. Zigmond. 1990. Chemoattractant-stimulated polymorphonuclear leukocytes contain two populations of actin filaments that differ in their spatial distributions and relative stabilities. J. Cell Biol. 110:1067–1075.k{\:, 百拇医药

    Chan, T.O., U. Rodeck, A.M. Chan, A.C. Kimmelman, S.E. Rittenhouse, G. Panayotou, and P.N. Tsichlis. 2002. Small GTPases and tyrosine kinases coregulate a molecular switch in the phosphoinositide 3-kinase regulatory subunit. Cancer Cell. 1:181–191.

    Chen, R., V.H. Kang, J. Chen, J.C. Shope, J. Torabinejad, D.B. DeWald, and G.D. Prestwich. 2002. A monoclonal antibody to visualize PtdIns(3,4,5)P3 in cells. J. Histochem. Cytochem. 50:697–708.u#e(r, 百拇医药

    Chung, C.Y., and R.A. Firtel. 1999. PAKa, a putative PAK family member, is required for cytokinesis and the regulation of the cytoskeleton in Dictyostelium discoideum cells during chemotaxis. J. Cell Biol. 147:559–576.u#e(r, 百拇医药

    Feig, L.A. 1999. Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases. Nat. Cell Biol. 1:E25–E27.u#e(r, 百拇医药

    Funamoto, S., R. Meili, S. Lee, L. Parry, and R.A. Firtel. 2002. Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis. Cell. 109:611–623.u#e(r, 百拇医药

    Haddad, E., J.L. Zugaza, F. Louache, N. Debili, C. Crouin, K. Schwarz, A. Fischer, W. Vainchenker, and J. Bertoglio. 2001. The interaction between Cdc42 and WASP is required for SDF-1-induced T-lymphocyte chemotaxis. Blood. 97:33–38.u#e(r, 百拇医药

    Hall, A. 1998. Rho GTPases and the actin cytoskeleton. Science. 279:509–514.

    Han, J., K. Luby-Phelps, B. Das, X. Shu, Y. Xia, R.D. Mosteller, U.M. Krishna, J.R. Falck, M.A. White, and D. Broek. 1998. Role of substrates and products of PI 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav. Science. 279:558–560.(d-m3[), http://www.100md.com

    Iijima, M., and P. Devreotes. 2002. Tumor suppressor PTEN mediates sensing of chemoattractant gradients. Cell. 109:599–610.(d-m3[), http://www.100md.com

    Just, I., J. Selzer, F. Hofmann, G.A. Green, and K. Aktories. 1996. Inactivation of Ras by Clostridium sordellii lethal toxin-catalyzed glucosylation. J. Biol. Chem. 271:10149–10153.(d-m3[), http://www.100md.com

    Kalman, D., S.N. Gomperts, S. Hardy, M. Kitamura, and J.M. Bishop. 1999a. Ras family GTPases control growth of astrocyte processes. Mol. Biol. Cell. 10:1665–1683.(d-m3[), http://www.100md.com

    Kalman, D., O.D. Weiner, D.L. Goosney, J.W. Sedat, B.B. Finlay, A. Abo, and J.M. Bishop. 1999b. Enteropathogenic E. coli acts through WASP and Arp2/3 complex to form actin pedestals. Nat. Cell Biol. 1:389–391.(d-m3[), http://www.100md.com

    Kraynov, V.S., C. Chamberlain, G.M. Bokoch, M.A. Schwartz, S. Slabaugh, and K.M. Hahn. 2000. Localized Rac activation dynamics visualized in living cells. Science. 290:333–337.

    Kroschewski, R., A. Hall, and I. Mellman. 1999. Cdc42 controls secretory and endocytic transport to the basolateral plasma membrane of MDCK cells. Nat. Cell Biol. 1:8–13.2f'.%o, 百拇医药

    Laudanna, C., D. Mochly-Rosen, T. Liron, G. Constantin, and E.C. Butcher. 1998. Evidence of zeta protein kinase C involvement in polymorphonuclear neutrophil integrin-dependent adhesion and chemotaxis. J. Biol. Chem. 273:30306–30315.2f'.%o, 百拇医药

    Manser, E., T. Leung, H. Salihuddin, Z.S. Zhao, and L. Lim. 1994. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature. 367:40–46.2f'.%o, 百拇医药

    Nern, A., and R.A. Arkowitz. 2000. G proteins mediate changes in cell shape by stabilizing the axis of polarity. Mol. Cell. 5:853–864.2f'.%o, 百拇医药

    Niggli, V. 2000. A membrane-permeant ester of phosphatidylinositol 3,4, 5-trisphosphate (PIP(3)) is an activator of human neutrophil migration. FEBS Lett. 473:217–221.2f'.%o, 百拇医药

    Nobes, C.D., and A. Hall. 1999. Rho GTPases control polarity, protrusion, and adhesion during cell movement. J. Cell Biol. 144:1235–1244.

    Ridley, A.J. 2001. Rho GTPases and cell migration. J. Cell Sci. 114:2713–2722.;v:z$, http://www.100md.com

    Roberts, A.W., C. Kim, L. Zhen, J.B. Lowe, R. Kapur, B. Petryniak, A. Spaetti, J.D. Pollock, J.B. Borneo, G.B. Bradford, et al. 1999. Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is characterized by abnormalities in neutrophil function and host defense. Immunity. 10:183–196.;v:z$, http://www.100md.com

    Sehr, P., G. Joseph, H. Genth, I. Just, E. Pick, and K. Aktories. 1998. Glucosylation and ADP ribosylation of rho proteins: effects on nucleotide binding, GTPase activity, and effector coupling. Biochemistry. 37:5296–5304.;v:z$, http://www.100md.com

    Servant, G., O.D. Weiner, E.R. Neptune, J.W. Sedat, and H.R. Bourne. 1999. Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis. Mol. Biol. Cell. 10:1163–1178.;v:z$, http://www.100md.com

    Servant, G., O.D. Weiner, P. Herzmark, T. Balla, J.W. Sedat, and H.R. Bourne. 2000. Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science. 287:1037–1040.;v:z$, http://www.100md.com

    Shinohara, M., Y. Terada, A. Iwamatsu, A. Shinohara, N. Mochizuki, M. Higuchi, Y. Gotoh, S. Ihara, S. Nagata, H. Itoh, et al. 2002. SWAP-70 is a guanine-nucleotide-exchange factor that mediates signalling of membrane ruffling. Nature. 416:759–763.

    Stephens, L.R., A. Eguinoa, H. Erdjument-Bromage, M. Lui, F. Cooke, J. Coadwell, A.S. Smrcka, M. Thelen, K. Cadwallader, P. Tempst, and P.T. Hawkins. 1997. The Gß{gamma})63u.nu, 百拇医药

    sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101. Cell. 89:105–114.)63u.nu, 百拇医药

    Symons, M., J.M. Derry, B. Karlak, S. Jiang, V. Lemahieu, F. McCormick, U. Francke, and A. Abo. 1996. Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization. Cell. 84:723–734.)63u.nu, 百拇医药

    Tapon, N., and A. Hall. 1997. Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton. Curr. Opin. Cell Biol. 9:86–92.)63u.nu, 百拇医药

    Tolias, K.F., J.H. Hartwig, H. Ishihara, Y. Shibasaki, L.C. Cantley, and C.L. Carpenter. 2000. Type Ialpha phosphatidylinositol-4-phosphate 5-kinase mediates Rac-dependent actin assembly. Curr. Biol. 10:153–156.)63u.nu, 百拇医药

    Verkhovsky, A.B., T.M. Svitkina, and G.G. Borisy. 1999. Self-polarization and directional motility of cytoplasm. Curr. Biol. 9:11–20.)63u.nu, 百拇医药

    Wang, F., P. Herzmark, O.D. Weiner, S. Srinivasan, G. Servant, and H.R. Bourne. 2002. Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils. Nat. Cell Biol. 4:513–518.]pq@|, 百拇医药

    Weiner, O.D. 2002. Regulation of cell polarity during eukaryotic chemotaxis: the chemotactic compass. Curr. Opin. Cell Biol. 14:196–202.]pq@|, 百拇医药

    Weiner, O.D., P.O. Neilsen, G.D. Prestwich, M.W. Kirschner, L.C. Cantley, and H.R. Bourne. 2002. A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity. Nat. Cell Biol. 4:509–513.]pq@|, 百拇医药

    Welch, H.C., W.J. Coadwell, C.D. Ellson, G.J. Ferguson, S.R. Andrews, H. Erdjument-Bromage, P. Tempst, P.T. Hawkins, and L.R. Stephens. 2002. P-Rex1, a PtdIns(3,4,5)P3- and Gbetagamma-regulated guanine-nucleotide exchange factor for Rac. Cell. 108:809–821.]pq@|, 百拇医药

    Zigmond, S.H., H.I. Levitsky, and B.J. Kreel. 1981. Cell polarity: an examination of its behavioral expression and its consequences for polymorphonuclear leukocyte chemotaxis. J. Cell Biol. 89:585–592.(Supriya Srinivasan Fei Wang Suzana Glavas Alexander Ott Fred Hofmann Klaus Aktories Daniel Kalman an)