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编号:10586066
Dual roles of the C-terminal Src kinase(Csk) during developmental vascularization
http://www.100md.com 《血液学杂志》2004年第4期
     From the Center for Vascular Biology, University of Connecticut Health Center, Farmington; Department of Cell Biology, University of Connecticut Health Center, Farmington; Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington; The Ben May Institute for Cancer Research and Center for Molecular Oncology, University of Chicago, Chicago, IL.{k, http://www.100md.com

    Here we report that C-terminal Src kinase (Csk), a tyrosine kinase that negatively regulates the activity of Src and related kinases, is important for vascular development. In Csk/embryos, although vascular tubules were formed and organized into capillary-like networks during the initial genesis of blood vessels, the vessels failed to engage in normal sprout formation. In chimeric embryos containing both wild-type and Csk/cells, the presence of wild-type cells enabled Csk/endothelial cells to participate in branching morphogenesis. We suggest that wild-type cells may have supplied an angiogenic factor absent in Csk/ cells. Despite the partial rescue of vascular development in chimeric embryos, the embryos failed to form vitelline vessels and died at E9.5. These results indicate that Csk is required both for angiogenic sprouting and vascular remodeling.(Li-Juan Duan Akira Imamoto and Guo-Hua Fong)