Jiang Hanying，Yu Yuan，Zhang Weijie，et al.

    Department of Clinical Pharmacdogy of Tongji Hospital Affiliated to Tongji Medical College Huazhong University of Science and Technology(HUST)，Wuhan430030.

    【Abstract】 Objective Greftt hombosis is the main Cause of early graft failure in discordant xenotranshplanˉtation.Altered endothelial cell(EC)production of thromboactive substance may play a primary role in this prodcedure.Methods To evaluate the potential role of nature antibodies and complement in hyperacut xenograft rejection we therefore investigated the effect of varing components of human serum on EC production of Prostacyclin(PGL2)apotent endogenous antagonis of platelet aggregation and vasodilator Confluent monolayers of pig aortic endothelial cell at stage three wereincubated for1or2hr in a Medium199contain0，10%fetal calf serum(FCS)，25%Mixed fresh human sera(HS)25%heatinactivated pooled humanserum(HIHS)，25%newborn sera.Results Test supernalant were then assayed by RIA for6-keto-PGFLa，the stable metabolite of PG12.The result of these experiment shown that fresh human serum inhibits PG12production and stimulates LDH release from PAECHowever ......