Soluble CD40 Ligand Levels Indicate Lipid Accumulation in Carotid Atheroma
Abstractn-!p^, 百拇医药
Objective— The CD40/CD40 ligand pathway mediates inflammatory processes important in atherogenesis and the formation of the intraplaque lipid pool. We tested the hypothesis that plasma levels of soluble CD40 ligand are elevated in patients with evidence of a lipid pool on high-resolution magnetic resonance imaging (MRI) of carotid stenoses.n-!p^, 百拇医药
Methods and Results— We recruited 49 patients with evidence of carotid atherosclerosis on ultrasonography; 3 patients could not undergo carotid MRI because of claustrophobia. The remaining 46 patients underwent high-resolution MRI of the carotid arteries. Two radiologists blinded to all other data determined the presence or absence of an intraplaque lipid pool based on the loss of signal between the 20-ms echo time (TE20) and the fat-suppressed TE55 fast spin-echo images. Plasma levels of soluble CD40 ligand were determined by ELISA. Baseline levels of soluble CD40 ligand were higher among patients with evidence of intraplaque lipid (n=14) than among those without it (n=32; median, 2.54 ng/mL; interquartile range [IQR], 1.85 to 3.52 vs median, 1.58 ng/mL; IQR, 1.21 to 2.39; P=0.02). In contrast, soluble CD40 ligand levels were not correlated with percent diameter stenosis (r=-0.19; P=0.21). The relative risk for intraplaque lipid associated with soluble CD40 ligand levels above the median was 6.0 (95% confidence interval, 1.15 to 31.23; P=0.03). The magnitude of this predictive effect did not substantially change after adjustment for traditional cardiovascular risk factors (relative risk, 5.12; 95% confidence interval, 0.78 to 33.73; P=0.09).
Conclusions— Plasma levels of soluble CD40 ligand may predict patients with features of high-risk atherosclerotic lesions. These data provide novel insight into the mechanism through which elevated levels of soluble CD40 ligand may reflect cardiovascular risk in humans and illustrate the potential value of interfacing high-resolution MRI with studies of vascular inflammation.i2x], http://www.100md.com
Key Words: CD40 ligand atherosclerosis lipid pool MRIi2x], http://www.100md.com
Introductioni2x], http://www.100md.com
The structure and dynamic biology of the atheroma, rather than the severity of stenosis, largely determine cardiovascular events. Large lipid pools and thin fibrous caps characterize vulnerable plaques, and inflammatory mechanisms play a pivotal role in determining plaque stability.1,2 Much of our knowledge of the unstable atheroma derives from postmortem examination, and less is known about the relationships of inflammatory mechanisms and lesion structure in vivo.
The binding of CD40 ligand with its receptor CD40 mediates many inflammatory responses important in atherosclerosis. A wide variety of inflammatory cells express CD40 ligand, and stimulation by other proinflammatory cytokines increases endothelial cell expression of CD40 ligand.3 Ligation of CD40 induces the expression of leukocyte adhesion molecules3 and triggers the release of chemoattractants overexpressed in human atheroma.4–6 Furthermore, CD40 ligation potently induces the expression of tissue factor,7,8 an important prothrombotic component of the intraplaque lipid pool. Thus, the spectrum of functions of CD40 ligand appears to span a wide range from early atherogenesis to late thrombotic complications.td, http://www.100md.com
Evidence from animal studies supports the importance of CD40 ligand because inhibition of CD40 signaling in atherosclerosis-prone mice reduced the size and lipid content of aortic lesions and yielded a relative increase in smooth muscle content and fibrillar collagen.9 Moreover, recent data show that elevated plasma levels of soluble CD40 ligand at baseline prospectively predict cardiovascular events among apparently healthy women.10
Recent advances in magnetic resonance imaging (MRI) have permitted noninvasive assessment of carotid plaque composition.11–13 Specifically, use of a custom-made, phased-array carotid coil has demonstrated high levels of agreement between carotid MRI findings and results of histological examination among 22 patients undergoing carotid endarterectomy (89% agreement; kappa=0.83; 95% confidence interval, 0.67 to 1.0).14 Furthermore, high-resolution carotid MRI accurately detects intraplaque lipid-rich cores.12,13,15,16vo)^, http://www.100md.com
This study tested the hypothesis that elevated plasma levels of soluble CD40 ligand are correlated with features suggestive of lipid-rich cores on high-resolution carotid MRI. For comparison, we also measured levels of soluble intercellular adhesion molecule-1 (sICAM-1), a marker of endothelial inflammation.vo)^, http://www.100md.com
Methodsvo)^, http://www.100md.com
Study Participantsvo)^, http://www.100md.com
From January 2001 to January 2002, we invited men and women with stenoses 30% in either the internal or common carotid artery as assessed by carotid ultrasonography to participate in the study. Any patient with a pacemaker or implantable cardioverter defibrillator was excluded, as well as patients who had received surgical clips or coronary stents in the previous 2 months. Patients with a systemic inflammatory condition or those requiring systemic corticosteroids were also excluded. The study population comprises the 49 consecutive patients who gave informed, written consent to participate in the study. The study was approved by the Human Research Committee of Brigham and Women’s Hospital.
A detailed medical history, including prior cardiovascular history, risk factors, and medication use, was recorded by a study physician for each participant. A blood sample was drawn by nontraumatic venipuncture and centrifuged, and the plasma was stored in EDTA at -80°C.7, http://www.100md.com
MRI Protocol7, http://www.100md.com
The patients underwent high-resolution MRI of the carotid arteries with a dedicated phased-array carotid coil (IGC, Inc) on a 1.5-T Signa CV/i MRI scanner (GE Medical Systems). Three-dimensional time-of-flight images, proton density–weighted images, and fat-suppressed T2-weighted images were obtained. For the 3D time-of-flight sequences, parameters were as follows: echo time (TE) 3.5 ms, repetition time (TR) 33 to 40 ms, flip angle 25°, bandwidth 15.63 kHz, field of view 12 to 14 cm, slice thickness 2 mm interpolated to 1 mm, 32 slices, acquired matrix 256x256, reconstructed matrix 512x512, and 1 excitation. For the proton density–weighted sequence, a TE of 21 to 22 ms was used, and for the T2-weighted acquisition, a TE of 53 to 58 ms was used with chemical-selective fat suppression. Parameters for these scans were as follows: TR of 2 R-R intervals, echo train length 16, bandwidth 62.5 kHz, field of view 14 cm, slice thickness 3 mm, acquired matrix 256x256, reconstructed matrix 512x512, and 1 excitation. Slice levels were centered at the carotid bifurcation in each patient. Two board-certified radiologists blinded to all other information determined the presence or absence of intraplaque lipid, based on the loss of signal between the proton density–weighted images and the fat-suppressed, T2-weighted fast spin-echo images with isointense signal on 3D time-of-flight imaging.15 The percent diameter stenosis was calculated as the difference between reference and stenotic diameters on 3D time-of-flight axial images, divided by the reference diameter and multiplied by 100. The images were read independently off-line. Interobserver variability was <10%.
Laboratory Studies#24, http://www.100md.com
Baseline plasma soluble CD40 ligand concentrations were measured by ELISA as previously described.10 In brief, 1:5-diluted plasma samples were applied in triplicate to 96-well plates precoated with mouse anti-human CD40 ligand antibody and mixed (1:2) with a horseradish peroxidase–labeled secondary mouse anti-human CD40 ligand antibody for 2 hours. Plates were then washed, and antibody binding was determined by colorimetry with 3,3'5,5'-tetramethyl benzidine substrate. Absorbance was read at 650 nm, and plasma concentrations of soluble CD40 ligand were determined by comparison with serial dilutions of recombinant human CD40 ligand. Intra-assay variation among the triplicates for all samples was <10%, and interassay variability was 7.4%. Plasma levels of sICAM-1 were determined by ELISA (R&D Systems).#24, http://www.100md.com
Statistical Analysis#24, http://www.100md.com
We divided the study participants into 2 groups, those with evidence of intraplaque lipid on carotid MRI and those without evidence of intraplaque lipid. Median levels of soluble CD40 ligand were computed and compared between the 2 groups with Wilcoxon’s ranked sum test. Relative risks of having intraplaque lipid associated with elevated soluble CD40 ligand levels were computed by use of logistic regression models that divided the study sample according to the median level of soluble CD40 ligand among those without evidence of intraplaque lipid.
Results.dy, 百拇医药
Of the 49 patients enrolled, carotid MR images could not be obtained for 3 patients because of claustrophobia. We report the results for the remaining 46 patients.shows the baseline clinical characteristics of the patients. There was a high prevalence of a history of hypertension, diabetes, and hypercholesterolemia in the overall study cohort. Thirteen of the 46 patients (28.3%) had a prior history of transient ischemic attack, (n=8) or stroke (n=6); 1 patient had suffered both a transient ischemic attack and a stroke. The remaining 33 patients (71.7%) were asymptomatic..dy, 百拇医药
fig.ommitted.dy, 百拇医药
. Baseline Clinical Characteristics of the Study Population.dy, 百拇医药
demonstrates the typical appearance of intraplaque lipid on proton density–weighted and fat-suppressed T2-weighted images. Fourteen patients had evidence of intraplaque lipid and 32 did not. Patients with evidence of intraplaque lipid more likely had diabetes (P=0.02) than those patients without evidence of intraplaque lipid. There was also a trend toward an increased proportion of women (P=0.1), patients with a history of hypertension (P=0.16), and current smokers (P=0.13) in the group with intraplaque lipid. Mean percent carotid diameter stenosis (58±20% vs 56±24%) did not differ between those with and without evidence of intraplaque lipid.
fig.ommitted0@, 百拇医药
Proton density–weighted (A) and fat-suppressed, T2-weighted (B) images showing a large lipid pool (arrow) in an internal carotid artery plaque.0@, 百拇医药
Subjects with intraplaque lipid had higher baseline levels of soluble CD40 ligand than those without lipid accumulations (median, 2.54 ng/mL; interquartile range [IQR], 1.85 to 3.52 vs median, 1.58 ng/mL; IQR, 1.21 to 2.39; P=0.02). In contrast, soluble CD40 ligand levels were not correlated with the percent diameter stenosis (r=-0.19; P=0.21). Plasma levels of sICAM-1 did not differ between those with intraplaque lipid and those without 0@, 百拇医药
Plasma CD40 levels tended to be higher among diabetic patients (n=13) compared with nondiabetics (n=33; 2.21 ng/mL, IQR, 1.66 to 2.77 vs 1.57 ng/mL; IQR, 1.16 to 2.48; P=0.14) and among women (n=15) compared with men (n=31) (2.31 ng/mL; IQR, 1.16 to 3.84 vs 1.72 ng/mL; IQR, 1.37 to 2.48; P=0.40), although these differences did not reach statistical significance. The relative risk for intraplaque lipid associated with soluble CD40 ligand levels above the median was 6.0 (95% confidence interval, 1.15 to 31.23; P=0.03). The magnitude of this predictive effect did not substantially change when analyzed by a multivariable model that controlled for the effects of sex, diabetes, hypertension, current smoking, percent stenosis, and ratio of total cholesterol to HDL cholesterol (relative risk, 5.12; 95% confidence interval, 0.78 to 33.73; P=0.09).
Discussion;zrzap, 百拇医药
Previous work has demonstrated that baseline plasma levels of soluble CD40 ligand prospectively predict cardiovascular events among apparently healthy women.10 The current data provide novel insight into the mechanism through which elevated levels of soluble CD40 ligand may reflect future cardiovascular risk in humans. We found an association between elevated plasma levels of soluble CD40 ligand and carotid plaques with features of high risk without relation to the severity of stenosis. These data agree with evidence from studies showing that interruption of CD40 signaling reduced the size and lipid content of aortic lesions in atherosclerosis-prone mice.9 In contrast, plasma levels of sICAM-1, a marker of endothelial inflammation, showed no relationship with the presence of carotid intraplaque lipid.;zrzap, 百拇医药
Our study has limitations. The cohort comprises a group of elderly patients with carotid atherosclerosis and a high prevalence of traditional risk factors. Thus, these data may not apply generally to other populations. We do not have histological confirmation of an intraplaque lipid pool. Nonetheless, previous work has found that high-resolution carotid MRI, using a similar phased-array carotid coil, can accurately predict histological findings of a lipid pool after carotid endarterectomy15; our MRI protocol relied more on T2-weighted protocols, which other studies have shown can accurately distinguish the lipid pool.12,13,16 It is possible that the potential confounding effect of diabetes or female sex, both of which tended to be associated with higher CD40 ligand levels in our study, or other unknown confounders may have affected the results of this cross-sectional study. However, we believe that this is unlikely, given that the magnitude of the predictive effect of soluble CD40 ligand persisted after adjustment for traditional cardiovascular risk factors, although the confidence intervals did widen in the adjusted analysis, as might be expected given that CD40 ligation appears to represent a common causal pathway in lipid pool formation. Finally, although imaging parameters were kept constant during this study, intrapatient variability in MR images could not be assessed with our study design.
In conclusion, we believe that this study establishes a link between plasma levels of CD40 ligand and intraplaque lipid, which represents 1 potentially important marker of plaque vulnerability. Further advances in plaque imaging, including reproducible measurement of fibrous cap thickness, may further enhance our understanding of the relationship between inflammation and plaque vulnerability. Interfacing studies of inflammation with high-resolution MRI may provide novel insights into the pathogenesis of atherosclerosis.\^9rj0, 百拇医药
Acknowledgments\^9rj0, 百拇医药
This study was supported by a young investigator grant from GlaxoSmithKline to Dr Blake and a grant from the Leducq Foundation, Paris; the SCOR program in molecular medicine and atherosclerosis from the National Heart, Lung, and Blood Institute (HL-48743); and a MERIT award from the National Institutes of Health to Dr Libby.\^9rj0, 百拇医药
References\^9rj0, 百拇医药
Davies MJ. Stability and instability: two faces of coronary atherosclerosis: the Paul Dudley White Lecture. Circulation. 1996: 94: 2013–2020.
Libby P. Molecular bases of the acute coronary syndromes. Circulation. 1995; 91: 2844–2850.$, 百拇医药
Karmann K, Hughes CC, Schechner J, Fanslow WC, Pober JS. CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression. Proc Natl Acad Sci U S A. 1995; 92: 4342–4346.$, 百拇医药
Kornbluth RS, Kee K, Richman DD. CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive ß-chemokines. Proc Natl Acad Sci U S A. 1998; 95: 5205–5210.$, 百拇医药
Mach F, Sauty A, Iarossi AS, Sukhova GK, Neote K, Libby P, Luster AD. Differential expression of three T lymphocyte-activating CXC chemokines by human atheroma-associated cells. J Clin Invest. 1999; 104: 1041–1050.$, 百拇医药
Denger S, Jahn L, Wende P, Watson L, Gerber SH, Kubler W, Kreuzer J. Expression of monocyte chemoattractant protein-1 cDNA in vascular smooth muscle cells: induction of the synthetic phenotype: a possible clue to SMC differentiation in the process of atherogenesis. Atherosclerosis. 1999; 144: 15–23.$, 百拇医药
Mach F, Schonbeck U, Bonnefoy JY, Pober JS, Libby P. Activation of monocyte/macrophage functions related to acute atheroma complication by ligation of CD40: induction of collagenase, stromelysin, and tissue factor. Circulation. 1997; 96: 396–399.
Schonbeck U, Mach F, Sukhova GK, Herman M, Graber P, Kehry MR, Libby P. CD40 ligation induces tissue factor expression in human vascular smooth muscle cells. Am J Pathol. 2000; 156: 7–14.!:, 百拇医药
Schonbeck U, Mach F, Sukhova GK, Atkinson E, Levesque E, Herman M, Graber P, Basset P, Libby P. Expression of stromelysin-3 in atherosclerotic lesions: regulation via CD40-CD40 ligand signaling in vitro and in vivo. J Exp Med. 1999; 189: 843–853.!:, 百拇医药
Schonbeck U, Varo N, Libby P, Buring J, Ridker PM. Soluble CD 40L and cardiovascular risk in women. Circulation. 2001; 104: 2266–2268.!:, 百拇医药
Fayad ZA, Fuster V. Clinical imaging of the high-risk or vulnerable atherosclerotic plaque. Circ Res. 2001; 89: 305–316.!:, 百拇医药
Shinnar M, Fallon JT, Wehrli S, Levin M, Dalmacy D, Fayad ZA, Badimon JJ, Harrington M, Harrington E, Fuster V. The diagnostic accuracy of ex vivo MRI for human atherosclerotic plaque characterization. Arterioscler Thromb Vasc Biol. 1999; 19: 2756–2761.!:, 百拇医药
Serfaty JM, Chaabane L, Tabib A, Chevallier JM, Briguet A, Douek PC. Atherosclerotic plaques: classification and characterization with T2-weighted high-spatial-resolution MR imaging: an in vitro study. Radiology. 2001; 219: 403–410.!:, 百拇医药
Hatsukami TS, Ross R, Polissar NL, Yuan C. Visualization of fibrous cap thickness and rupture in human atherosclerotic carotid plaque in vivo with high-resolution magnetic resonance imaging. Circulation. 2000; 102: 959–964.!:, 百拇医药
Yuan C, Mitsumori LM, Ferguson MS, Polissar NL, Echelard D, Ortiz G, Small R, Davies JW, Kerwin WS, Hatsukami TS. In vivo accuracy of multispectral magnetic resonance imaging for identifying lipid-rich necrotic cores and intraplaque hemorrhage in advanced human carotid plaques. Circulation. 2001; 104: 2051–2056.!:, 百拇医药
Toussaint JF, Southern JF, Fuster V, Kantor HL. T2-weighted contrast for NMR characterization of human atherosclerosis. Arterioscler Thromb Vasc Biol. 1995; 15: 1533–1542.(Gavin J. Blake Robert J. Ostfeld E. Kent Yucel Nerea Varo Uwe Schönbeck Michael A. Blake Marie )
Objective— The CD40/CD40 ligand pathway mediates inflammatory processes important in atherogenesis and the formation of the intraplaque lipid pool. We tested the hypothesis that plasma levels of soluble CD40 ligand are elevated in patients with evidence of a lipid pool on high-resolution magnetic resonance imaging (MRI) of carotid stenoses.n-!p^, 百拇医药
Methods and Results— We recruited 49 patients with evidence of carotid atherosclerosis on ultrasonography; 3 patients could not undergo carotid MRI because of claustrophobia. The remaining 46 patients underwent high-resolution MRI of the carotid arteries. Two radiologists blinded to all other data determined the presence or absence of an intraplaque lipid pool based on the loss of signal between the 20-ms echo time (TE20) and the fat-suppressed TE55 fast spin-echo images. Plasma levels of soluble CD40 ligand were determined by ELISA. Baseline levels of soluble CD40 ligand were higher among patients with evidence of intraplaque lipid (n=14) than among those without it (n=32; median, 2.54 ng/mL; interquartile range [IQR], 1.85 to 3.52 vs median, 1.58 ng/mL; IQR, 1.21 to 2.39; P=0.02). In contrast, soluble CD40 ligand levels were not correlated with percent diameter stenosis (r=-0.19; P=0.21). The relative risk for intraplaque lipid associated with soluble CD40 ligand levels above the median was 6.0 (95% confidence interval, 1.15 to 31.23; P=0.03). The magnitude of this predictive effect did not substantially change after adjustment for traditional cardiovascular risk factors (relative risk, 5.12; 95% confidence interval, 0.78 to 33.73; P=0.09).
Conclusions— Plasma levels of soluble CD40 ligand may predict patients with features of high-risk atherosclerotic lesions. These data provide novel insight into the mechanism through which elevated levels of soluble CD40 ligand may reflect cardiovascular risk in humans and illustrate the potential value of interfacing high-resolution MRI with studies of vascular inflammation.i2x], http://www.100md.com
Key Words: CD40 ligand atherosclerosis lipid pool MRIi2x], http://www.100md.com
Introductioni2x], http://www.100md.com
The structure and dynamic biology of the atheroma, rather than the severity of stenosis, largely determine cardiovascular events. Large lipid pools and thin fibrous caps characterize vulnerable plaques, and inflammatory mechanisms play a pivotal role in determining plaque stability.1,2 Much of our knowledge of the unstable atheroma derives from postmortem examination, and less is known about the relationships of inflammatory mechanisms and lesion structure in vivo.
The binding of CD40 ligand with its receptor CD40 mediates many inflammatory responses important in atherosclerosis. A wide variety of inflammatory cells express CD40 ligand, and stimulation by other proinflammatory cytokines increases endothelial cell expression of CD40 ligand.3 Ligation of CD40 induces the expression of leukocyte adhesion molecules3 and triggers the release of chemoattractants overexpressed in human atheroma.4–6 Furthermore, CD40 ligation potently induces the expression of tissue factor,7,8 an important prothrombotic component of the intraplaque lipid pool. Thus, the spectrum of functions of CD40 ligand appears to span a wide range from early atherogenesis to late thrombotic complications.td, http://www.100md.com
Evidence from animal studies supports the importance of CD40 ligand because inhibition of CD40 signaling in atherosclerosis-prone mice reduced the size and lipid content of aortic lesions and yielded a relative increase in smooth muscle content and fibrillar collagen.9 Moreover, recent data show that elevated plasma levels of soluble CD40 ligand at baseline prospectively predict cardiovascular events among apparently healthy women.10
Recent advances in magnetic resonance imaging (MRI) have permitted noninvasive assessment of carotid plaque composition.11–13 Specifically, use of a custom-made, phased-array carotid coil has demonstrated high levels of agreement between carotid MRI findings and results of histological examination among 22 patients undergoing carotid endarterectomy (89% agreement; kappa=0.83; 95% confidence interval, 0.67 to 1.0).14 Furthermore, high-resolution carotid MRI accurately detects intraplaque lipid-rich cores.12,13,15,16vo)^, http://www.100md.com
This study tested the hypothesis that elevated plasma levels of soluble CD40 ligand are correlated with features suggestive of lipid-rich cores on high-resolution carotid MRI. For comparison, we also measured levels of soluble intercellular adhesion molecule-1 (sICAM-1), a marker of endothelial inflammation.vo)^, http://www.100md.com
Methodsvo)^, http://www.100md.com
Study Participantsvo)^, http://www.100md.com
From January 2001 to January 2002, we invited men and women with stenoses 30% in either the internal or common carotid artery as assessed by carotid ultrasonography to participate in the study. Any patient with a pacemaker or implantable cardioverter defibrillator was excluded, as well as patients who had received surgical clips or coronary stents in the previous 2 months. Patients with a systemic inflammatory condition or those requiring systemic corticosteroids were also excluded. The study population comprises the 49 consecutive patients who gave informed, written consent to participate in the study. The study was approved by the Human Research Committee of Brigham and Women’s Hospital.
A detailed medical history, including prior cardiovascular history, risk factors, and medication use, was recorded by a study physician for each participant. A blood sample was drawn by nontraumatic venipuncture and centrifuged, and the plasma was stored in EDTA at -80°C.7, http://www.100md.com
MRI Protocol7, http://www.100md.com
The patients underwent high-resolution MRI of the carotid arteries with a dedicated phased-array carotid coil (IGC, Inc) on a 1.5-T Signa CV/i MRI scanner (GE Medical Systems). Three-dimensional time-of-flight images, proton density–weighted images, and fat-suppressed T2-weighted images were obtained. For the 3D time-of-flight sequences, parameters were as follows: echo time (TE) 3.5 ms, repetition time (TR) 33 to 40 ms, flip angle 25°, bandwidth 15.63 kHz, field of view 12 to 14 cm, slice thickness 2 mm interpolated to 1 mm, 32 slices, acquired matrix 256x256, reconstructed matrix 512x512, and 1 excitation. For the proton density–weighted sequence, a TE of 21 to 22 ms was used, and for the T2-weighted acquisition, a TE of 53 to 58 ms was used with chemical-selective fat suppression. Parameters for these scans were as follows: TR of 2 R-R intervals, echo train length 16, bandwidth 62.5 kHz, field of view 14 cm, slice thickness 3 mm, acquired matrix 256x256, reconstructed matrix 512x512, and 1 excitation. Slice levels were centered at the carotid bifurcation in each patient. Two board-certified radiologists blinded to all other information determined the presence or absence of intraplaque lipid, based on the loss of signal between the proton density–weighted images and the fat-suppressed, T2-weighted fast spin-echo images with isointense signal on 3D time-of-flight imaging.15 The percent diameter stenosis was calculated as the difference between reference and stenotic diameters on 3D time-of-flight axial images, divided by the reference diameter and multiplied by 100. The images were read independently off-line. Interobserver variability was <10%.
Laboratory Studies#24, http://www.100md.com
Baseline plasma soluble CD40 ligand concentrations were measured by ELISA as previously described.10 In brief, 1:5-diluted plasma samples were applied in triplicate to 96-well plates precoated with mouse anti-human CD40 ligand antibody and mixed (1:2) with a horseradish peroxidase–labeled secondary mouse anti-human CD40 ligand antibody for 2 hours. Plates were then washed, and antibody binding was determined by colorimetry with 3,3'5,5'-tetramethyl benzidine substrate. Absorbance was read at 650 nm, and plasma concentrations of soluble CD40 ligand were determined by comparison with serial dilutions of recombinant human CD40 ligand. Intra-assay variation among the triplicates for all samples was <10%, and interassay variability was 7.4%. Plasma levels of sICAM-1 were determined by ELISA (R&D Systems).#24, http://www.100md.com
Statistical Analysis#24, http://www.100md.com
We divided the study participants into 2 groups, those with evidence of intraplaque lipid on carotid MRI and those without evidence of intraplaque lipid. Median levels of soluble CD40 ligand were computed and compared between the 2 groups with Wilcoxon’s ranked sum test. Relative risks of having intraplaque lipid associated with elevated soluble CD40 ligand levels were computed by use of logistic regression models that divided the study sample according to the median level of soluble CD40 ligand among those without evidence of intraplaque lipid.
Results.dy, 百拇医药
Of the 49 patients enrolled, carotid MR images could not be obtained for 3 patients because of claustrophobia. We report the results for the remaining 46 patients.shows the baseline clinical characteristics of the patients. There was a high prevalence of a history of hypertension, diabetes, and hypercholesterolemia in the overall study cohort. Thirteen of the 46 patients (28.3%) had a prior history of transient ischemic attack, (n=8) or stroke (n=6); 1 patient had suffered both a transient ischemic attack and a stroke. The remaining 33 patients (71.7%) were asymptomatic..dy, 百拇医药
fig.ommitted.dy, 百拇医药
. Baseline Clinical Characteristics of the Study Population.dy, 百拇医药
demonstrates the typical appearance of intraplaque lipid on proton density–weighted and fat-suppressed T2-weighted images. Fourteen patients had evidence of intraplaque lipid and 32 did not. Patients with evidence of intraplaque lipid more likely had diabetes (P=0.02) than those patients without evidence of intraplaque lipid. There was also a trend toward an increased proportion of women (P=0.1), patients with a history of hypertension (P=0.16), and current smokers (P=0.13) in the group with intraplaque lipid. Mean percent carotid diameter stenosis (58±20% vs 56±24%) did not differ between those with and without evidence of intraplaque lipid.
fig.ommitted0@, 百拇医药
Proton density–weighted (A) and fat-suppressed, T2-weighted (B) images showing a large lipid pool (arrow) in an internal carotid artery plaque.0@, 百拇医药
Subjects with intraplaque lipid had higher baseline levels of soluble CD40 ligand than those without lipid accumulations (median, 2.54 ng/mL; interquartile range [IQR], 1.85 to 3.52 vs median, 1.58 ng/mL; IQR, 1.21 to 2.39; P=0.02). In contrast, soluble CD40 ligand levels were not correlated with the percent diameter stenosis (r=-0.19; P=0.21). Plasma levels of sICAM-1 did not differ between those with intraplaque lipid and those without 0@, 百拇医药
Plasma CD40 levels tended to be higher among diabetic patients (n=13) compared with nondiabetics (n=33; 2.21 ng/mL, IQR, 1.66 to 2.77 vs 1.57 ng/mL; IQR, 1.16 to 2.48; P=0.14) and among women (n=15) compared with men (n=31) (2.31 ng/mL; IQR, 1.16 to 3.84 vs 1.72 ng/mL; IQR, 1.37 to 2.48; P=0.40), although these differences did not reach statistical significance. The relative risk for intraplaque lipid associated with soluble CD40 ligand levels above the median was 6.0 (95% confidence interval, 1.15 to 31.23; P=0.03). The magnitude of this predictive effect did not substantially change when analyzed by a multivariable model that controlled for the effects of sex, diabetes, hypertension, current smoking, percent stenosis, and ratio of total cholesterol to HDL cholesterol (relative risk, 5.12; 95% confidence interval, 0.78 to 33.73; P=0.09).
Discussion;zrzap, 百拇医药
Previous work has demonstrated that baseline plasma levels of soluble CD40 ligand prospectively predict cardiovascular events among apparently healthy women.10 The current data provide novel insight into the mechanism through which elevated levels of soluble CD40 ligand may reflect future cardiovascular risk in humans. We found an association between elevated plasma levels of soluble CD40 ligand and carotid plaques with features of high risk without relation to the severity of stenosis. These data agree with evidence from studies showing that interruption of CD40 signaling reduced the size and lipid content of aortic lesions in atherosclerosis-prone mice.9 In contrast, plasma levels of sICAM-1, a marker of endothelial inflammation, showed no relationship with the presence of carotid intraplaque lipid.;zrzap, 百拇医药
Our study has limitations. The cohort comprises a group of elderly patients with carotid atherosclerosis and a high prevalence of traditional risk factors. Thus, these data may not apply generally to other populations. We do not have histological confirmation of an intraplaque lipid pool. Nonetheless, previous work has found that high-resolution carotid MRI, using a similar phased-array carotid coil, can accurately predict histological findings of a lipid pool after carotid endarterectomy15; our MRI protocol relied more on T2-weighted protocols, which other studies have shown can accurately distinguish the lipid pool.12,13,16 It is possible that the potential confounding effect of diabetes or female sex, both of which tended to be associated with higher CD40 ligand levels in our study, or other unknown confounders may have affected the results of this cross-sectional study. However, we believe that this is unlikely, given that the magnitude of the predictive effect of soluble CD40 ligand persisted after adjustment for traditional cardiovascular risk factors, although the confidence intervals did widen in the adjusted analysis, as might be expected given that CD40 ligation appears to represent a common causal pathway in lipid pool formation. Finally, although imaging parameters were kept constant during this study, intrapatient variability in MR images could not be assessed with our study design.
In conclusion, we believe that this study establishes a link between plasma levels of CD40 ligand and intraplaque lipid, which represents 1 potentially important marker of plaque vulnerability. Further advances in plaque imaging, including reproducible measurement of fibrous cap thickness, may further enhance our understanding of the relationship between inflammation and plaque vulnerability. Interfacing studies of inflammation with high-resolution MRI may provide novel insights into the pathogenesis of atherosclerosis.\^9rj0, 百拇医药
Acknowledgments\^9rj0, 百拇医药
This study was supported by a young investigator grant from GlaxoSmithKline to Dr Blake and a grant from the Leducq Foundation, Paris; the SCOR program in molecular medicine and atherosclerosis from the National Heart, Lung, and Blood Institute (HL-48743); and a MERIT award from the National Institutes of Health to Dr Libby.\^9rj0, 百拇医药
References\^9rj0, 百拇医药
Davies MJ. Stability and instability: two faces of coronary atherosclerosis: the Paul Dudley White Lecture. Circulation. 1996: 94: 2013–2020.
Libby P. Molecular bases of the acute coronary syndromes. Circulation. 1995; 91: 2844–2850.$, 百拇医药
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