【摘要】 目的 观察榄香烯抑制神经胶质瘤细胞体外增殖的量效、时效关系。方法 采用细胞计数、MTT、H 3 -TdR、集落形成试验等方法分别检测了不同细胞密度、不同药物浓度和不同作用时间榄香烯对胶质瘤细胞的作用效果。结果 榄香烯对C6、U251和SHG-44胶质瘤细胞均具有明显的抑制增殖作用。相比之下，在相同药物浓度鼠源性胶质瘤细胞C6较人源性胶质瘤细胞U251及SHG-44敏感。榄香烯对胶质瘤细胞的增殖抑制效应呈剂量依赖性，随着药物浓度和药物作用时间的增加，抑制效应增强。榄香烯能显著抑制胶质瘤细胞集落形成，当浓度达到20mg/L～40mg/L以上时，几乎完全抑制了肿瘤细胞克隆形成。结论 榄香烯能有效抑制胶质瘤细胞的体外恶性增殖。

    关键词 胶质瘤 榄香烯 增殖

    【文献标识码】 A 【文章编号】1681-102X(2004)03-0193-03

    Elemene inhibits the proliferation of glioma cells in vitro

    Zhou Hongyu，Wang Yong，Jiang Jiyao，et al.

    Department of Neurosurgery，Renji Hospital，Shanghai Second Medical University，Shanghai200001.

    【Abstract】 Objective To determine the proliferation inhibitory effect of elemene on glioma cells in a time and concentration-dependent relationship in vitro.Methods Rat C6glioma cells and human SHG-44，U251glioma cells were incubated under various elemene concentrations.The effects of elemene on cell proliferation were studied by using cells counting，MTT assay，H 3 -TdR and colony-forming efficiency(CFE)in double-layer soft agar.Results Elemene caused a marked dose-dependent and time-dependent proliferation inhibitory effect on C6 ......