【摘要】 目的 研究模拟飞行器离心机训练对原代培养的兔主动脉内皮细胞新的第二信使内源性一氧化碳(CO)及其合成酶血红素氧化酶-1(HO-1)的作用。方法 通过反转录聚合酶链式反应、斑点杂交、放免法及生物化学等多项指标观察模拟飞行离心机训练对培养主动脉内皮细胞中新的第二信使CO及其合成酶HO-1的作用。结果 模拟飞行离心机训练能刺激培养主动脉内皮细胞HO-1的表达及CO含量的增高，也能刺激主动脉内皮细胞ET-1mRNA表达增高。但加入氯化高铁血红素后，HO-1mRNA的表达增高，产生更多的CO及cGMP，从而导致ET-1mRNA表达减低。结论 增强HO-1mRNA的表达及CO含量能降低由模拟飞行离心机训练诱导的作用最强的血管收缩肽ET-1mRNA的表达。

    Yu Lu，Luo Huilan

    Department of Kidney，305Hospital of People Liberation Army，Beijing100017.

    【Abstract】 Objective To research the interaction of the simution fling centrifuge training with the new secˉond message-endogenous carbon monoxide and its synzyme heme oxygenase-1in primary culture rabbit vascular enˉdothelial cell.Methods Reverse transcription-polymerse chain reaction，dot blot and some biochemical index marks were performed so as to research the interaction of the simution fling centrifuge training with endogenous carbon monoxide and his synzyme heme oxygenase-1.Results The simution fling centrifuge training can incite the express of heme oxygenase-1mRNA and increase the concentration of endogenous carbon monoxide and incite the express of ET-1mRNA.After adding hemin ......