【摘要】 目的 最近研究表明，Ras-MAPK信号转导通路在肝癌的发生和发展中起着重要作用，p38MAPK是Ras-MAPK信号转导通路中的一关键分子。本研究拟探讨p38MAPK在肝癌发病中的作用。方法 用脂质体转染法将survivin反义寡核苷酸(antisense oligodeoxynucleotide，ASO)导入肝癌细胞系SMMC-7721细胞中，通过细胞凋亡、p38MAPK含量、p38MAPK表达和p38MAPK活性的变化检测p38MAPK在肝癌发病中的作用。结果 survivin反义寡核苷酸作用SMMC-7721细胞48h后凋亡细胞比率、p38MAPK含量、p38MAPK表达和p38MAPK活性分别为对照组的5.3倍、4.6倍、5.1倍和6.3倍，差异有显著性(P<0.05)。结论 p38MAPK通过介导凋亡信号而在肝癌的发病中起重要作用。

    关键词 肝癌 反义寡核苷酸 丝裂原活化的蛋白激酶 信号转导

    The role of p38mitogen-activated protein kinase in the pathogenesis of liver cancer

    Song Tingting，Li Miaoyu，Jiang Yuhua，et al.

    Department of Recuperation，Qingdao Tumor Hospital，Shandong266042

    【Abstract】 Objective To study of the role of p38mitogen-activated protein kinase(p38MAPK)in the pathoˉgenesis of liver cancer.Methods After survivin antisense oligodeoxynucleotide(ASO)was introduced into SMMC-7721cell line by liposomal transfection，the effect was evaluated by apoptosis，p38MAPK content，p38MAPK expression and p38MAPK activity.Results The rate of apoptotic cells，p38MAPK content，p38MAPK expression and p38MAPK activity were4.3，3.6 ......