【摘要】 目的 探讨三苯氧胺诱导乳腺癌细胞凋亡可能的机制。 方法 用流式细胞仪检测细胞凋亡，四甲基偶氮唑蓝(MTT)比色法测定细胞增殖活性。用RT-PCR和Western-blot检测Survivin mRNA和蛋白表达。 结果 TAM浓度在1μmol时，发挥最大的促凋亡和抑增殖活性，且Survivin mRNA蛋白表达下降最明显。 结论 三苯氧胺诱导乳腺癌细胞凋亡与Survivin有关。

    【关键词】 三苯氧胺 乳腺癌 细胞凋亡 Survivin

    The mechanism studies of apoptosis induced by tamoxifen in breast cancer

    Li Junchuan，Xie Ping，Chen Tingxuan，et al.

    Departement of Pathology，The First Pepole's Hospital of Jingzhou，Hubei434000.

    【Abstract】 Objective To investigate mechanism of apoptosis induced by tamoxifen in breast cancer.Meth-ods Detecting apoptosis by flow cytometry and measuring cell proliferation activity by MTT assary in breast cancino-ma.Expression of survivin mRNA protein was evaluated by RT-PCR and western blot analysis.Results When ta-moxifen was1?mol，promoting apoptosis，inhibiting proliferation activity and survivin mRNA protein expression singnificantly decreasing was detected in the human breast carcinoma cell line.Conclusion Apoptosis induced by ta- moxifen in breast cancer has been associated with survivin playing important pole in regulation of cell cycle. ......