【摘要】 目的 观察山楂叶总黄酮对小鼠组织细胞脂蛋白脂酶表达影响。 方法 将小鼠随机分为正常对照组、吡咯列酮组、山楂叶总黄酮组(n=10)，给药8天后，ELISA法检测血液、脂肪组织、肌组织所含LPL。 结果 与对照组相比，各组鼠血液中LPL均无明显改变(P>0.05);肌组织中吡咯列酮组LPL增高(P<0.05)，山楂叶总黄酮高、中、低组的LPL增高(P<0.001、P<0.01);而脂肪组织中吡咯列酮组的LPL降低(P<0.05)，山楂叶总黄酮高、中组的LPL降低(P<0.01)。 结论 山楂叶总黄酮能影响小鼠LPL的表达，并有组织表达差异。

    【关键词】 脂蛋白脂酶 山楂叶总黄酮 吡咯列酮 酶联免疫法

    Effect of hawthorn total flavonoids on expression of lipoprotein lipase in mice tissues

    Yan Jin，Fan Chunlei，Wo Xingde，et al.

    Life Science Department，Zhejiang College of Traditional Chinese Medicine，Hangzhou310053.

    【Abstract】 Objective To investigate the effect of total flavonoids of hawthorn leaves on the expression of lipoprotein lipase in mice tissues.Methods 50ICR mice were randomly divided into three-groups:the control group was treated with distilled water;pioglitazone group was treated with10mg/kg;the flavonoids groups were treated with16mg/kg，8mg/kg，4mg/kg respectively.The LPL mass in serum，adipose tissue，muscular tissue of each group were detected by enzyme labeling linked immunosorbent assay(ELISA).Results No significant difference of LPL mass in serum was found for all groups.Comparing with the control group ......