基金项目:本课题由江苏省教委产业化项目(JH01-066)

    【摘要】 目的 将人组织激肽释放酶(基因命名为KLK1，酶命名为hK1)cDNA克隆入大肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株。方法 将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。结果 经用方法证明，本研究研制的单克隆抗体还能与鸡输卵管表达的重组hK1发生特异反应。结论 这些试验结果表明，本研究制备的单克隆抗体能用于hK1的分子特性研究。

    关键词 人激肽释放酶单克隆抗体 鉴定

    Preparation and identification of a monoclonal antibody specific

    for humantissue kallikrein

    Zhang Lei，Sun Huaichang，Wang Anping，et al.

    Jiangsu Provincial Center of Animal Transgenesis and Biopharning，Yangzhou University，Yangzhou225009.

    【Abstract】 Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate i_ˉdentification of human tissue kallikrein(KLK1gene，hK1protein).Methods Mice were immunized with E.coli-ex_ˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.In addition ......