P34cdc2﹑CyclinB1在大肠癌中的表达及意义
颜文娟, 邓长生,武汉大学医学院消化内科 湖北省武汉市 430071
通讯作者:颜文娟, 430071, 湖北省武汉市, 武汉大学医学院消化内科. whywj2005@163.com
电话: 027-87330224
收稿日期: 2005-01-06 接受日期: 2005-02-26
摘要
目的:研究p34cdc2、细胞周期素B1(cyclin B1)在大肠癌组织中的表达，并探讨其与大肠癌发生、发展的关系.
, http://www.100md.com
方法:应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶法检测p34cdc2、cyclin B1在64例大肠腺癌组织及10例正常对照大肠组织中的表达情况.
结果:64例大肠癌组织中p34cdc2、cyclin B1表达阳性率分别为68.75%和82.81%，定位于细胞质中，呈棕黄色或棕褐色.10例正常对照大肠组织中p34cdc2、cyclin B1部分为弱阳性及中度阳性表达.病例组与对照组比较差异均有显著性(P<0.05).cyclin B1与p34cdc2的表达呈正相关，相关系数为0.586.p34cdc2和cyclinB1在大肠癌组织中的表达在有淋巴结和远隔器官转移组与没有淋巴结和远隔器官转移组间其差异有显著性(P<0.05);而在根据患者年龄、性别、肿瘤的原发部位、分化程度等进行分组的组间其表达的差异没有显著性.在大肠癌组织中PCNA阳性细胞呈弥漫分布，呈明显异质性，阳性率为73.44%.在正常大肠组织中见PCNA阴性或少量阳性表达.经统计学分析在大肠癌组织中PCNA的表达与p34cdc2和cyclin B1均没有相关性.
, 百拇医药
结论:p34cdc2、cyclin B1在大肠癌中呈过表达，可作为反映大肠癌淋巴结转移和远隔器官转移的指标之一.
颜文娟, 邓长生. P34cdc2、Cyclin B1在大肠癌中的表达及意义. 世界华人消化杂志 2005;13(10):1228-1230
Solorzano L, Rieber MS, Rieber M. Lower cyclin H and cyclin-dependent kinase-activating kinase activity in cell cycle
arrest induced by lack of adhesion to substratum. Cancer Res 2000;60:7114-7118
, http://www.100md.com
2 Seong J, Chung EJ, Kim H, Kim GE, Kim NK, Sohn SK, Min JS, Suh CO. Assessment of biomarkers in paired primary
and recurrent colorectal adenocarcinomas. Int J Radiat Oncol Biol Phys 1999;45:1167-1173
3 Deng J, Zhou J, Meng F, Li D, Sun H. Failure to inactivate CDK activity is responsible for the enhanced apoptotic response
in U937 cells mediated by silencing ATM gene. J Huazhong Univ Sci Technolog Med Sci 2002;22:193-196
, 百拇医药
4 O'Connor DS, Grossman D, Plescia J, Zhang H, Villa A, Tognin S, Marchisio PC, Altieri DC. Regulation of apoptosis at
cell division by p34cdc2 phosphorylation of survivin. Proc Natl Acad Sci USA 2000;97:13103-13107
5 Nozoe T, Honda M, Inutsuka S, Korenaga D. P34cdc2 expression is an independent indicator for lymph node metastasis
in colorectal carcinoma. J Cancer Res Clin Oncol 2003;129:498-502
, http://www.100md.com
6 刘标, 陈小毅, 苟新敏. 鼻咽良恶性病变中EBV-LMP1, Cyclin B1, p34cdc2 和Survivin 的表达及意义.
广东医学院学报 2004;22:10-12
7 Yasuda M, Takesue F, Inutsuka S, Honda M, Nozoe T, Korenaga D. Overexpression of cyclin B1 in gastric cancer and
its clinicopathological significance:an immunohistological study. J Cancer Res Clin Oncol 2002;128:412-416
8 Kourea HP, Koutras AK, Scopa CD, Marangos MN, Tzoraco-eleftherakis E, Koukouras D, Kalofonos HP. Expression
, http://www.100md.com
of the cell cycle regulatory protein p34cdc2, p21waf1, and p53 in node negative invasive ductal breast carcinoma.
Mol Pathol 2003;56:328-335
9 Blagsklonny MV, Pardee AB. Exploiting cancer cell cycling for selective protection of normal cells.
Cancer Res 2001;61:4301-4305
编辑 张海宁, 百拇医药( 颜文娟,邓长生)
通讯作者:颜文娟, 430071, 湖北省武汉市, 武汉大学医学院消化内科. whywj2005@163.com
电话: 027-87330224
收稿日期: 2005-01-06 接受日期: 2005-02-26
摘要
目的:研究p34cdc2、细胞周期素B1(cyclin B1)在大肠癌组织中的表达，并探讨其与大肠癌发生、发展的关系.
, http://www.100md.com
方法:应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶法检测p34cdc2、cyclin B1在64例大肠腺癌组织及10例正常对照大肠组织中的表达情况.
结果:64例大肠癌组织中p34cdc2、cyclin B1表达阳性率分别为68.75%和82.81%，定位于细胞质中，呈棕黄色或棕褐色.10例正常对照大肠组织中p34cdc2、cyclin B1部分为弱阳性及中度阳性表达.病例组与对照组比较差异均有显著性(P<0.05).cyclin B1与p34cdc2的表达呈正相关，相关系数为0.586.p34cdc2和cyclinB1在大肠癌组织中的表达在有淋巴结和远隔器官转移组与没有淋巴结和远隔器官转移组间其差异有显著性(P<0.05);而在根据患者年龄、性别、肿瘤的原发部位、分化程度等进行分组的组间其表达的差异没有显著性.在大肠癌组织中PCNA阳性细胞呈弥漫分布，呈明显异质性，阳性率为73.44%.在正常大肠组织中见PCNA阴性或少量阳性表达.经统计学分析在大肠癌组织中PCNA的表达与p34cdc2和cyclin B1均没有相关性.
, 百拇医药
结论:p34cdc2、cyclin B1在大肠癌中呈过表达，可作为反映大肠癌淋巴结转移和远隔器官转移的指标之一.
颜文娟, 邓长生. P34cdc2、Cyclin B1在大肠癌中的表达及意义. 世界华人消化杂志 2005;13(10):1228-1230
Solorzano L, Rieber MS, Rieber M. Lower cyclin H and cyclin-dependent kinase-activating kinase activity in cell cycle
arrest induced by lack of adhesion to substratum. Cancer Res 2000;60:7114-7118
, http://www.100md.com
2 Seong J, Chung EJ, Kim H, Kim GE, Kim NK, Sohn SK, Min JS, Suh CO. Assessment of biomarkers in paired primary
and recurrent colorectal adenocarcinomas. Int J Radiat Oncol Biol Phys 1999;45:1167-1173
3 Deng J, Zhou J, Meng F, Li D, Sun H. Failure to inactivate CDK activity is responsible for the enhanced apoptotic response
in U937 cells mediated by silencing ATM gene. J Huazhong Univ Sci Technolog Med Sci 2002;22:193-196
, 百拇医药
4 O'Connor DS, Grossman D, Plescia J, Zhang H, Villa A, Tognin S, Marchisio PC, Altieri DC. Regulation of apoptosis at
cell division by p34cdc2 phosphorylation of survivin. Proc Natl Acad Sci USA 2000;97:13103-13107
5 Nozoe T, Honda M, Inutsuka S, Korenaga D. P34cdc2 expression is an independent indicator for lymph node metastasis
in colorectal carcinoma. J Cancer Res Clin Oncol 2003;129:498-502
, http://www.100md.com
6 刘标, 陈小毅, 苟新敏. 鼻咽良恶性病变中EBV-LMP1, Cyclin B1, p34cdc2 和Survivin 的表达及意义.
广东医学院学报 2004;22:10-12
7 Yasuda M, Takesue F, Inutsuka S, Honda M, Nozoe T, Korenaga D. Overexpression of cyclin B1 in gastric cancer and
its clinicopathological significance:an immunohistological study. J Cancer Res Clin Oncol 2002;128:412-416
8 Kourea HP, Koutras AK, Scopa CD, Marangos MN, Tzoraco-eleftherakis E, Koukouras D, Kalofonos HP. Expression
, http://www.100md.com
of the cell cycle regulatory protein p34cdc2, p21waf1, and p53 in node negative invasive ductal breast carcinoma.
Mol Pathol 2003;56:328-335
9 Blagsklonny MV, Pardee AB. Exploiting cancer cell cycling for selective protection of normal cells.
Cancer Res 2001;61:4301-4305
编辑 张海宁, 百拇医药( 颜文娟,邓长生)
