林志彬 林志彬(北京大学 基础医学院药理学系，北京100083) 基础医学与临床 2000 0 20 5


    关键词： 期刊 jcyxylc 0 中药现代药理专题 fur -->


    

中图分类号：R282.71 文献标识码：A

    文章编号：1001-6325(2000)05-0007-03

Abstract: Ganoderma lucidum(Leyss .Ex fr .)Karst .(Lingzhi) is a medicinal fungus with a long history in China as avaluable tonic remedy .Modern Pharmacological andclinical investigation demonstrated that Lingzhi had anti-tumor activities .Recently the antitumor effects of extract of Ganoderma lucidum(GLE)and Ganoderma polysaccharides B(GL-B) and its mechanism were investigated .The results demonstrated that GLE and GL-B significantly inhibitedgrowth of implanted sarcoma 180 in vivo .GL-B alsopromoted anti-tumor activity induced by cyclophosphamide in mice in vivo .GLE and GL-B directly adding to tumor cells-cultured medium neithersuppressed sarcoma 180 and HL-60 proliferation nor induced apoptosis of both tumor cellsin vitro .However the serum from GLE and GL-B treatedmice can suppress S-180 and HL-60 cells proliferation and induced its apoptosis in vitro .Furthermore splenocytes conditioned medium with GL-B (GL-B-S-CM)and peritoneal macrophages conditional medium with GL-B (GL-B-PM-CM) also significantlyinhibited HL-60 proliferation and induced its apoptosis in vitro .Further study indicated that GLE and GL-B could promote TNFaproduction from murine peritoneal macrophages and IFNg production from murine spleen cellsin vitro .Finally GLE and GL-B could promote TNFamRNA expression in murine peritoneal macrophages and IFNg mRNA expression in murine spleencells .The results suggest that the anti-tumor effectof Ganoderma lucidum relates to activating macrophage and spleen cell ......