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关键词：米非司酮；卵巢肿瘤；Ki-67抗原；免疫组化；流式细胞术
摘要 目的：探讨米非司酮对人卵巢上皮性癌细胞的 杀伤活性及对细胞增殖和细胞周期分布的影响。方 法：采用MTT测定，观察不同浓度米非司酮(20、10、5、 2.5μmol/L)对HO-8910细胞的杀伤活性；通过ABC免疫组化 染色及流式细胞仪分析，检测用药后HO-8910细胞Ki-67抗 原表达、细胞周期分布及细胞增殖指数的变化。结 果：4个浓度的米非司酮对HO-8910细胞均有杀伤作用， 并呈剂量依赖性，以20μmol/L浓度杀伤作用最明显，杀 伤率达52.01%；5μmol/L米非司酮可使HO-8910细胞Ki-67抗 原表达显著减少，细胞增殖受到明显抑制，使细胞 阻滞于G0/G1期，不能进入S及G2/M期，细胞增殖指数明显 下降。结论：米非司酮体外对人卵巢上皮性癌细胞 具有杀伤活性，并对细胞增殖及细胞周期有明显抑 制作用，主要通过抑制DNA合成，将细胞阻滞于G0/G1期 。
Effects of Mifepristone on Cell Proliferation and Cell Cycle Kinetics of Human Ovarian Epithelial Carcinoma Cell Line HO- 8910 in Vitro
Sun Lijun Liu Huifan Guo Haizhou
(Second Hospital of Henan Medical University, Zhengzhou)
Abstract Objective: To study the effects of mifepristone on cell proliferation and cell cycle kinetics of human ovarian carcinoma cell lines HO- 8910 in vitro. Methods: The antiproliferation effects of four different concentrations of mifepristone (20,10,5,2.5μ mol/L) on HO- 8910 cell line were determined with MTT assay. Immunohistochemical (ABC) and flow cytometry techniques were used to determine the changes of Ki- 67 antigen expression and cell cycle distribution and cell proliferation index after treatment with mifepristone. Results: Four different concentrations dose- dependently inhibited the proliferation of HO- 8910 cells. The inhibition by mifepristone plateaued at 20μ mol/L (52% inhibition). Mifepristone at 5μ mol/L decreased the expression of Ki- 67 antigen of HO- 8910 cells. It (5μ mol/L) caused a decline in the proportion of cells in S and G2/M phase, blocked the cell cycle progression in G0/G1 phase and reduced the proliferation index (PI) of HO- 8910 cells. Conclusion: In vitro, Mifepristone significantly inhibites the growth of human ovarian epithelial carcinoma cells which are progestin receptor positive and leads to arrest of the cell cycle progression in G0/G1 phase.
Key Words Mifepristone Ovarian neoplasm Immunohistochemistry Ki- 67 antigen Flow cytometry
性激素与某些肿瘤的发生发展有密切关系 ......
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