吴晓华 高奉浔 陈俐 吴晓华 第三军医大学大坪医院野战外科研究所病理科(重庆市 400042)；高奉浔 第三军医大学大坪医院野战外科研究所病理科(重庆市 400042)；陈俐 第三军医大学大坪医院野战外科研究所病理科(重庆市 400042) 中国肿瘤临床 2000 0 27 4


    关键词：大肠癌；基底膜；免疫组化；浸润转移 期刊 zgzllc 0 285-288 论著 fur -->


    

摘要 目的 ：检测基底膜主要成分层粘连蛋白(LN)、纤维粘结蛋白(FN)及Ⅳ型胶原纤维(ColⅣ)在大肠癌中的表达以及与浸润、转移的关系。方法 ：免疫组化标记大肠癌组织中ColⅣ、LN及FN。结果 ：大肠癌组织中基底膜成分存在不同程度的缺失，表现为ColⅣ、LN呈不连续、片状分布；癌组织分化越低，LN缺失越明显；ColⅣ表达与组织类型有显著关系，而与分化程度无关。一般腺癌阳性率明显高于粘液腺癌及印戒细胞癌；DukesB、C期大肠癌ColⅣ表达阳性率低于A期大肠癌；FN表达与上述因素关系不大。结论 ：大肠癌基底膜成分的缺失与其侵袭、转移能力有关，尤其是ColⅣ的缺失可能标志其具较强的侵袭、转移能力。

    Expressionof Laminin,Fibronectin and Collagen Ⅳ

    in Colorectal Carcinomas

Wu Xiaohua Gao Fengxun Chen Li

    (Daping Hospital/Research Institute of Surgery, The Third Military Medical University,Chongqing)

Abstract Purpose : This studysought to determine if there is a correlation between the expression of major componentsof basement membrane such as collagen Ⅳ , fibronectin, laminin etc. and the invasion andmetastasis of colorectal carcinomas. Methods : An immunohistochemicalstudy of the major basement membrane antigen collagen Ⅳ , fibronectin and laminin wasperformed and 109 cases of colorectal carcinoma were observed. Results :There was a statistically significant correlation between the collagen Ⅳ expression andhistologic type of colorectal carcinoma. The depletion of collagen Ⅳ was more prominentin mucinous carcinoma and signet carcinoma than that in adenocarcinoma, but no closecorrelation was found between the expression of collagen Ⅳ and the histologic stage oftumor. Only about 30% of the stage B and stage C tumors expressed collagen Ⅳ . Thispercentage was slightly lower than that of stage A tumors. In addition, laminin orfibronectin expression did not show any significant association with these parametersmentioned above. Conclusion : The absence of the components of basementmembrane especially the collagen Ⅳ is intimately associated with invasion and metastasisin the case of colorectal carcinomas.

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