聂时南 李兆申 许国铭 湛先保 屠振兴 龚燕芳 段义民 崔忠敏 刘枫 聂时南(第二军医大学长海医院消化内科，上海 200433)；李兆申(第二军医大学长海医院消化内科，上海 200433)；许国铭(第二军医大学长海医院消化内科，上海 200433)；湛先保(第二军医大学长海医院消化内科，上海 200433)；屠振兴(第二军医大学长海医院消化内科，上海 200433)；龚燕芳(第二军医大学长海医院消化内科，上海 200433)段义民(第二军医大学长海医院消化内科，上海 200433)；崔忠敏(第二军医大学长海医院消化内科，上海 200433) 第二军医大学学报 2000 0 21 1


    关键词：一氧化氮合酶；胃粘膜；应激 期刊 dejydxxb 0 专题报道：胃粘膜应激损伤研究 fur -->


    

摘 要： 目的:探讨躯体性及心理性应激状态下大鼠胃粘膜NOS活性变化及其与胃粘膜损伤的关系。方法：将96只雄性SD大鼠随机分为3组：对照组，规则光(R)组及不规则光(I)组，采用高压恒流脉冲刺激器制备躯体性和心理性应激模型。分光光度法检测胃粘膜组织的NOS活性和Nils Lambecht法测定胃粘膜损伤指数。结果:正常情况下NOS活性平稳，应激后大鼠胃粘膜NOS活性均明显升高，I组升高程度更明显；与对照组比较，R,I组胃粘膜损伤指数均较高，且I组较R组变化显著(P<0.05)。结论：心理性应激程度愈高，胃粘膜损伤愈严重，胃粘膜损伤程度及NOS活性变化与心理应激程度关系密切。

    中图分类号： R 573.1 文献标识码： A

    文章编号： 0258-879X(2000)01-0010-03

Relation between the activity change of nitric oxide synthase (NOS) and acute gastric mucosal lesion in gastric mucosa under physical and psychological stress in rats

NIE Shi-Nan

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    LI Zhao-Shen

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    XU Guo-Ming

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    ZHAN Xian-Bao

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    TU Zhen-Xing

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    GONG Yan-Fang

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    DUAN Yi-Min

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    CUI Zhong-Ming

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

    LIU Feng

    (Department of Gastroenterology,Changhai Hospital,Second Military Medical University, Shanghai 200433, China)

ABSTRACT： Objective: To explore the relation between the activity changes of nitric oxide synthase and acute gastric mucosal lesion in gastric mucosa under physical and psychological stress. Methods: Animal model was constructed with impulsive stimulator of high voltage and stable currency. Ninety-six male SD rats were randomly divided into 3 groups: control, regular and irregular group. NOS activity was measured by spectrophotometry and injure of gastric mucosa was measured by method of Nils Lambecht. Results: NOS activity was stable in normal rats but increased significantly in group of psychological stress(P<0.05); gastric mucosa injury were observed in regular group, irregular group and the later was server(P<0.05). Conclusion: The degree of increase of the NOS activity and gastric mucosa injury are positively correlated to the severity of psychological stress.

    KEY WORDS ： nitric oxide synthase; gastric mucosal; stress▲

[Acad J Sec Mil Med Univ, 2000, 21(1): 10-12]

一氧化氮合酶(NOS)广泛存在于胃粘膜中 ......