【摘要】 目的 探讨内皮素、NO同自体静脉移植后狭窄的关系。方法 对26例自体静脉移植后狭窄并进行2次手术者以及正常对照组26例，利用放射免疫测定、Griss方法检测血浆内皮素和NO水平，利用反转录-聚合酶链反应检测狭窄血管组织内皮素和一氧化氮合酶(NOS)基因表达水平。结果 所有患者均存在高内皮素、低NO血症，存在狭窄血管组织内皮素基因高表达、NOS基因低表达状态。结论 内皮素、NO同自体静脉移植再狭窄密切相关。

Relationship between nitric oxide, endothelins and autograft vein stricture

WANG Chunxi ,DUAN Zhiquan ,WU Xianzhong ,et al .

    (The First Hospital ,China Medical University ,Shengyang 110001)

【Abstract】 Objective To investigate the relationship between endothelins-1 (ET-1), nitric oxide (NO) and autograft vein stricture.Methods The levels of ET-1, NO and message ribonucleic acid (mRNA) gene expression of ET-1, NO synthase (NOS) were determined by using radioimmunoassay, Griss method and reverse transcription polymerase chain reaction (RT-PCR) respectively. Results All patients had high level of ET-1 and high gene expression of ET-1 mRNA, had low level of NO and low gene expressionn of NOS. Conclusion The abnormalities of ET-1, NO plasma levels and gene expression of ET-1 mRNA, NOS mRNA were closely related with autograft vein stricture.

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