摘要 目的：探讨星形细胞瘤(ASA)细胞谱系型别及其与生物学行为和病理学分级的关系。方法：免疫组化方法60例人脑ASA的A2B5、GFAP及GC抗原表达和谱系分型。结果：45例ASA为1型谱系星形细胞瘤(T1LA)；15例ASA含较高密度的A2B5+细胞。为2型谱系星形细胞瘤(T2LA)。复发性肿瘤A2B5标记指数显著高于非复发性病例，而GFAP标记指数呈现相反的结果。T2LA复发率显著高于T1LA(P<0.02)。12例ASA含有低密度的GC+细胞。结论：T1LA比T2LA常见；以A2B5和GFAP对ASA进行谱系分型，有利于其诊断和复发性估计。GC标记利于识别星形-少枝混合性胶质细胞瘤。

Abstract Objective: To investigate the relationships between the cell lineages and different histopathologic grade. Methods: Using A2B5 monoclonal antibody, anti-GFAP monoclonal antibody, and anti-galactocerebroside (GC) polyclonal antibody with an immunohistochemistry (ABC method) to detect the expression of these antigens, 60 astrocytomas were examined. Results: 45 astrocytomas relatively lacked A2B5 antigen and shared the same lineage as type 1 astrocytes. 15 astrocytomas appeared to represent neoplasms with A2B5 positive lineage, thereby sharing the same lineage as type 2 astrocytes. The recurrence cases demonstrated significantly higher A2B5 label index and significantly lower GFAP label index than those of the nonrecurrence cases (P <0.02 and 0.01). And type 2 lineage tumors (T2LA) presented significantly higher recurrence rate than that of type 1 lineage tumors (T1LA) (P<0.02). 12 astrocytomas contained low densities of GC positive cells. Conclusions: T2LA is less common than T1LA. The lineage types using immunohistochemical analysis with A2B5 and GFAP may be useful in diagnosis and in predicting the recurrence ability of astrocytomas. GC label is useful in diagnosis of mixed oligo-astrocytomas.

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