王景昌 王庆余 陈幸华 彭贤贵 630038 重庆，第三军医大学新桥医院[王景昌(现在解放军第十医院)、王庆余、陈幸华、彭贤贵] 中华血液学杂志 1999 0 20 1


    关键词：白细胞介素3；白血病，髓样，急性；柔红霉素 期刊 zhxyxzz 0 论著 fur -->


    

【摘要】 目的 观察经重组人白细胞介素3(rhIL-3)刺激后急性髓系白血病(AML)细胞对柔红霉素(DNR)敏感性的变化。方法 用流式细胞仪、细胞形态学、联苯胺染色观察rhIL-3对AML细胞增殖与分化的影响;用荧光分光光度法测定AML细胞内DNR浓度;用半固体培养法观察CFU-AML产率。结果 ①rhIL-3对体外培养的AML细胞具有促增殖作用,但无明显诱导分化作用; ②rhIL-3不影响AML细胞对DNR的摄取与排泄平衡,但能增强AML细胞对DNR的敏感性。结论 rhIL-3与DNR联合应用,有可能提高临床治疗AML的疗效。

Study on the sensitization of acute myeloid leukemia cell to daunorubicin by recombinant human interleukin-3 WANG Jingchang, WANG Qingyu,CHEN Xinghua, et al.Department of Hematology,Xin Qiao Hospital,Third Military Medical University, Chongqing 630038

     【 Abstract】 Objective To evaluate the effect of recombinant human interleukin-3(rhIL-3) on the sensitization of acute myeloid leukemia(AML) cells to daunorubicin(DNR).Methods AML cells were cultured in a DNR-containing medium supplemented or not with rhIL-3. CFU-AML was assayed by semi-solid culture, proliferation and differentiation of AML cells were assayed by flow cytometry, cytomorphology and benzidine staining.Intracellular DNR concentration was measured by spectrophotofluorometry.Results rhIL-3 could enhance the proliferation but not differentiation of AML cells in vitro, and had no effect on DNR intake and exclusion of AML cells while enhancing their sensitivity to DNR. Conclusion rhIL-3 combined with DNR might improve the therapeutic effect of AML.

     【 Key words】 Interleukin-3 Leukemia, myeloid, acute Daunorubicin

细胞动力学研究表明,白血病细胞存在较多的非增殖期(G₀ )或增殖较慢的细胞 ......