摘要 目的: 探讨多发性骨髓瘤(MM)患者骨髓细胞中白细胞介素6(IL-6)分泌机制。方法: 应用双标记免疫荧光技术检测了16例MM患者和19例对照者新鲜分离的骨髓单个核细胞中IL-6表达情况。结果: MM患者骨髓单个核细胞IL-6表达阳性率为15.4%±6.5%,骨髓瘤细胞IL-6表达阳性率达7.9%±2.9%,基质细胞IL-6表达阳性率达4.5%±1.2%;对照组骨髓单个核细胞IL-6表达阳性率为4.6%±2.3%,基质细胞IL-6表达阳性率为2.6%±1.1%,浆细胞IL-6表达阳性率为0.7%±0.1%。MM患者均较对照组显著升高(P均<0.001)。另外MM组骨髓单个核细胞中基质细胞所占比率为6.9%±1.4%,也较对照组的4.7%±1.8%明显升高(P<0.001)。结论: MM患者升高的IL-6既可由瘤细胞分泌,又可由基质细胞分泌,自分泌和旁分泌机制可能都参与了MM的发病。

    Mechanism of IL-6 secretion in bone marrow cells from multiple myeloma patients Liang Zhixiang^* ,Hou Jian,Ding Siqi,et al.^* Changzheng Hospital,Second Military Medical University,Shanghai 200003

     Abstract Objective: To explore the mechanism of interleukin-6(IL-6) secretion in bone marrow cells from patients with multiple myeloma(MM).Methods: Expression of IL-6 in bone marrow mononuclear cells(BMMNC) from MM patients(n=16) and controls(n=19)was detected by using double labelled immunofluorescence.Results: In MM group,the positive rates of IL-6 expression were 15.4%±6.5% in BMMNC,7.9%±2.9% in myeloma cells,and 4.5%±1.2% in stromal cells,while in controls the positive rates were 4.6%±2.3% in BMMNC,2.6%±1.1% in stromal cells and 0.7%±0.1% in plasma cells,respectively.The positive rates in MM group were all significantly higher than those in controls(P<0.001).The cytoplasmic IgG⁺ and vWF⁺ cells percentages in BMMNC were also significantly higher in MM group than those in controls.Conclusion: Elevated IL-6 in MM patients was secreted by both myeloma cells and stromal cells,and the mechanism of both autocrine and paracrine may be involved in pathogeneses of multiple myeloma.

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