摘要 目的 ：定量检测急性淋巴细胞白血病化疗效应差异及其临床意义。方法 ：应用竞争性PCR技术，定量检测急性淋巴细胞白血病(ALL)细胞中T细胞受体γ(TCRγ)基因重排。结果 ：检测ALL患者54例158份骨髓标本，随访时间中位数为33.5个月。临床缓解时36例患者的白血病细胞<1×10^-5 ，18例为(354±327)×10^-5 。诱导缓解期的杀伤效应与缓解早期的残留白血病细胞数量显著相关，并与临床复发相关。部分患者缓解后残留白血病细胞数量持续小于1×10^-5 且临床无复发。结论 ：白血病化疗效应差异显著，化疗效应的定量评价有助于制定个体化的治疗方案。

Detection of TCRγ gene in acute lymphoblastic leukemia after remission and itsclinical significance Liu Leyu, Sun Jinying, Yin Huijun. Pediatric Department of People's Hospital,Beijing Medical University,Beijing 100044

    Abstract Objectives:To investigate the quantitative evaluation of chemotherapy efficiency in patients withacute leukemia and its clinical significance. Methods: Clonal rearrangement of T-cellreceptor γ gene in ALL was detected quantitatively byusing competitive PCR. Results: One hundred and fifty-eight samples were collected from 54childhood ALL. The median follow-up duration was 33.5 months. At remission, the amount ofleukemic cells in 36 cases was less than 1×10^-5 , and in 18, was (354±327)×10^-5 .The efficiency of induction therapy was related significantly to the amount of thedetectable residual leukemic cells in the early remission phase and to clinical relapse.In some patients in remission,the amount of leukemic cells persistently under 1×10^-5 without clinical relapse. Conclusion: Quantitative evaluation of the chemotherapyefficiency is helpful for individualized treatment.

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