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丙型肝炎病毒高变区基因序列的比较研究
http://www.100md.com 《中华肝脏病杂志》 1999年第1期
丙型肝炎病毒高变区基因变异,关键词:
     潘文胜 王海涛 汪涛 郭华章 骆抗先 100071,北京,军事医学科学微生物研究所(潘文胜,王海涛); 广州南方医院传染科(骆抗先); 第四军医大学(汪涛,郭华章) 中华肝脏病杂志 1999 0 7 1


    关键词:丙型肝炎病毒 高变区 基因变异 期刊 zhgzbzz 0 临床研究 fur -->


    

摘要 目的 探索丙型肝炎预后的规律。方法 用RT-PCR方法从1例丙型肝炎病毒(HCV)持续感染者和1例HCV感染恢复者血清中扩增出HCV高变区基因片段,并用标准的分子生物学方法对PCR产物进行多个克隆的序列分析。结果 两者在突变位点的百分率、核苷酸类似株(quasispecies)的多样性、与国内HCVⅡ型参考株的同源性及各克隆间的遗传距离等方面差异均无显著性。在HCV感染恢复者所测得的20个克隆中有2个克隆1个碱基的插入突变,而在HCV持续感染者的高变区16个克隆中没有发现此现象。结论 HCV高变区基因变异程度似乎与HCV感染后恢复与否无必然联系。表明在考虑基因变异的同时,还需考虑其它因素,才能判断基因变异与预后的关系。

    

Comparison of hypervariable region gene of hepatitis C virus between an individual infected persistently and an individual recovered from infection

PAN Wensheng,WANG Haitao,WANG Tao,et al.Institute of Microbiology and Epidemiology,Beijing 100071

Abstract Objective To explore the law of recovery from HCV infection.Methods Amplifiying,sequenceing and analyzing the gene of hypervariable region from an individual infected persistently and an individual recovered from infection.Results One base insertion (two clones,10%) in the person recovered other than that infected persistently,but there is no difference between them in percentage of mumation,diversity of quasispecies and genetic distance among clones.Conclusion The range of genetic variation at one time point did not seem to have obvious relationship with the HCV persistent infection or recovery from HCV infection.This study gave basic information for searching the relation of genetic variation and clinical status,and designing HCV vaccine.

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