王佑春 庄辉 张华远 李倬 毛群颍 刘欣 Ling R Harrison TJ 李河民 100050,北京,中国药品生物制品检定所(王佑春,张华远,毛群颖,李河民); 北京医科大学微生物系(庄辉); 北京市佑安医院(李倬); 河南省肝病研究所(刘欣); Royal Free Hospital School of Medicine,London(Ling R.Harrison TJ) 中华肝脏病杂志 1999 0 7 1


    关键词：戊型肝炎病毒 变异株 期刊 zhgzbzz 0 临床研究 fur -->


    

摘要 目的 探讨戊型肝炎病毒(HEV)患者血清学特征。方法 用逆转录聚合酶链反应法(RT-PCR)检测HEV RNA，并对阳性产物进行克隆和测序；对部分HEVRNA阳性者进行追踪并检测抗-HEV。结果 15例(23.4%)为HEV RNA阳性，其中9例为典型的中国HEV株基因序列，6例变异较大，与典型的中国株基因序列的同源性仅为80%左右。感染原型HEV株的4例中，3例于1月后抗-HEV阳转；而2例感染变异HEV株的患者于1月或3月时，抗-HEV仍为阴性。结论 现行的抗-HEV EIA试剂尚不能诊断变异的HEV株。

    

Serological character of hepatitisE virus mutants

WANG Youchun,ZHUANG Hui,ZHANGHuayuan,et al.National Institute for the Control of Pharmaceutical and BiologicalProducts,Beijing 100050

Abstract Objective 64sera with serologically negative nonA-E were tested for HEV RNA and their serologicalcharacteristics were studied.Methods HEV RNA was tested by a reversetranscriptase PCR(RT-PCR) and the positive PCR products were cloned and sequenced.Of 15patients positive for HEV RNA,6 were followed up and tested for anti-HEV.Results 15 of them(23.4%) were HEV RNA positive.9 of the 15 HEV sequences have 95% homology andthe remaining 6 have only 80% homology as compared with the Chinese HEV strain at thenucleotide level.Of 15 patients positive for HEV RNA,6 were followed up.3 of 4 patientsinfected with typical HEV had anti-HEV seroconversion 1 month after infection and 2patients infected with HEV mutants were persistently negative for anti-HEV 1 or 3 monthsafter infection.Conclusion The Commercial anti-HEV EIA Kit can not detectHEV mutants.

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