金宇 郭世绂 赵虬 雪原 管力 金宇 067000承德医学院附属医院骨科；郭世绂、赵虬、雪原 天津医科大学总医院骨科；管力 承德医学院 中华骨科杂志 1999 0 19 2


    关键词：脊髓损伤；低温；移植 期刊 zhgkzz 0 脊柱脊髓疾患 fur -->


    

【摘要】目的 建立了一个胚胎脊髓(fetal spinal cord)组织的低温保存方法和一个损伤移植模式。方法 通过病理学、组织化学和免疫组织化学的研究,选用胎龄14天的Wistar大鼠，分离胚胎脊髓，逐步降温，最后置于液氮中保存。15天后取出，快速复温，并植入损伤大鼠脊髓内。全部动物于伤后8周处死。结果 经过低温保存的移植物能够在异体脊髓内存活并与宿主组织融合，其在损伤移植区的胶质细胞增生少于对照组。移植组宿主再生神经纤维能穿越宿主—移植物界面进入移植区。结论 经冰冻保存的胚胎脊髓移植物改变了宿主的再生环境，并显示对损伤轴突有营救作用。

    Experimental Transplantation of Fetal Spinal Cord and Observation of Its Influence to the Injured Spinal Cord in Rat

JIN Yu^* ， GUO Shifu, ZHAO Qiu,et al.

    ^* Department of Orthopaedics, Affiliated Hospital of Chengde Medical College, Chengde 067000

【 Abstract 】 Objective To establish a method of cryopreservation of fetal spinal cord ( FSC) tissue and a model of spinal cord injury for the transplantation. Methods FSC tissue was harvested form E14 Wistar rats and cut into slices of 1 cubic mm. It was put into protective liquid for cryopreservation in liquid nitrogen( - 195.8 ℃ ). Spinal cord injury( SCI) model was produced in 24 wistar rats at T ₇ level using modified Allen’ s impact method with 50 g^. cm .The animals were divided into transplanted and control group randomly. After 4 weeks, cryopreserved FSC was grafted into injured segments in the transplanted group. All animals were sacrificed at 8 weeks following the impact injury. The pathologic ......