赵少平 任龙喜 马铁鹏 五爱民 陈志坚 尹佳丽 063000 河北省唐山市第二医院 中华手外科杂志 1999 0 15 4


    关键词：腕骨；月骨；缺血性坏死 期刊 zhswkzz 0 月骨无菌性坏死 fur -->


    

【摘要】 目的 研究人发角蛋白人工肌腱(human-hair keratin artificial tendon, HHKAT)的组织相容性、抗原性及其在上肢肌腱修复术或功能重建术中的应用。 方法 取18只Wistar大白鼠，将HHKAT(实验组)及相同重量的正常人发(对照组)，分别埋植至大鼠脊柱两侧皮下。 术后2、8和12周取材，比较两种材料的组织学反应。 临床应用HHKAT 14例，另选25例为对照组。 术前、术后分别取其外周血，测定免疫球蛋白IgG,IgA,IgM,补体C3，T淋巴细胞亚群以及T淋巴细胞转化，并对其结果进行比较。 结果 实验结果证实HHKAT的组织相容性较好，对组织的刺激性小于正常人发( P < 0.05)。 14例术前、术后的免疫学指标无明显差异(P > 0.05)，与对照组相比也无明显差异(P > 0.05)；证实HHKAT对人体无明显的抗原性。 患者切口均一期愈合，术后随访13 ～ 25个月，功能与病种及肌力评定，患肢功能恢复优良率达92.8 %。 结论 HHKAT为目前较为理想的一种人工肌腱代用品。

Immunological study on human - hair keratin artificial tendon

    and the preliminary results of its clinical application

ZOU Yunwen, WANG Zhijie, JI Aiyu, et al. Department of Orthopedic Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003

【Abstract】 Objective To investigate the biocompatibility and antigenity of human-hair keratin artificial tendon (HHKAT) and evaluate its application in repair of tendons of the upper extremities.Methods HHKAT and normal human hair were implanted in the subcutaneous tissue at the two sides of the spine in 18 Wistar rats. Se pre - and postoperative immunological paramaters of patients who received human - hair keratin artificial tendon transplantation had no statistically significant difference (P > 0.05). There was no difference between patients of HHKAT group and control group (P > 0.05). All the wounds healed primarily. The excellent and good rate of the function after 13 to 25 months’ follow - up was 92.8 %. Conclusions HHKAT has no significant antigenity to the human body. It is an ideal substitute for tendon material at the moment.

     ......