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支气管哮喘淋巴细胞Fas抗原的表达
http://www.100md.com 《中华结核和呼吸感染》 1998年第4期
哮喘|糖皮质激素类,关键词:
支气管哮喘淋巴细胞Fas抗原的表达

     张劭夫 刘树芬 张波 徐军 高长兴 250031 济南,济南军区总医院呼吸科(张劭夫、刘树芬、张波、徐军);解放军第54871部队(高长兴) 中华结核和呼吸感染 1998 0 0 4


    关键词:哮喘;糖皮质激素类 期刊 zhjhhhxgr 0 论著 fur -->


    

摘要 目的 探讨哮喘患者淋巴细胞Fas抗原的表达状况。方法 取哮喘患者外周血淋巴细胞,加入地塞米松10-5 mol/L进行细胞培养,分别于0、2、8、24小时以流式细胞仪测定淋巴细胞表面的Fas(CD95 )抗原。结果 哮喘患者淋巴细胞Fas抗原的自发表达较正常对照组低,哮喘组Fas为5.3% ± 20%,正常组为8.5% ± 1.8%,(P<0.05)。体外经地塞米松诱导后其Fas抗原表达显著增加(P<0.01)。结论 哮喘患者免疫亢进可能与免疫细胞Fas抗原自发性降低有关。地塞米松可诱导淋巴细胞Fas抗原的高表达,可能是地塞米松诱导细胞凋亡的机理之一。

The expression ofFas antigen on lymphocytes in the patients with asthma

Zhang Shaofu, Liu Shufen,Zhang Bo, et al. Respiration Department, Gerenal Hospital of Jinan Command of PLA, Jinan250031

Abstract Objective To study the expression of Fas antigen on lymphocytes in patientswith asthma. Method Theperipheral blood lymphocytes (PBL) isolated from patients with asthma were cultured withdexamethasone 10-5 mol/L, Fas antigen on lymphocytes was detected by flowcytometry at 0, 2 nd, 8 th, 24th hour respectively. Result The native expression of Fas antigen of PBL isolated from thepatients with asthma (5.3% ± 2.0%, P<0.05) was lower than that fromhealthy controls (8.5% ± 1.8%). Increased expression of Fas antigen in thepatients with asthma was observed with induction of dexamethasone (P<0.01). Thepercentages of Fas positive cell were 5.3% ± 2.0%, 33% ± 5%, 63% ± 11%,76% ± 7% at 0, 2nd, 8 th, 24 th hour respectively inasthmatic group. While 8.5% ± 1.8%, 31% ± 11%, 49% ± 10%,20% ± 4% respectively in normal control group. Conclusion Decreased native expression of Fas on lymphocytes may berelated to airway inflammation in the patients with asthma. Increased expression of Fasinduced by Dex may be one of the mechanism of lymphocytes apoptosis.

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