史皆然 孙滨 战兴利 710032 西安，第四军医大学西京医院呼吸内科 中华结核和呼吸感染 1998 12 21 12


    关键词：白细胞介素；单克隆抗体；哮喘；动物，实验 期刊 zhjhhhxgr 0 论 著 fur -->


    

【摘要 】 目的 初步观察抗白细胞介素5(IL-5)单抗对哮喘豚鼠嗜酸性粒细胞(EOS)的影响。方法 用鸡卵清蛋白(OVA)腹腔注射法复制豚鼠哮喘模型，随机分为两组，每组15只，联合应用雾化吸入和腹腔注射法分别给予生理盐水(NS)及抗IL-5单克隆抗体(McAb)治疗，比较基础、治疗前、后外周血(PB)和支气管肺泡灌洗液(BALF)中EOS的改变。结果 McAb治疗组豚鼠外周血EOS计数治疗前[(1.76±0.27)×10⁹ /L]与治疗后[(1.63±0.26)×10⁹ /L]比较，差异有显著性(P<0.05)，而NS组则无明显改变(P>0.05)；治疗后McAb组BALF中EOS数[(0.41±0.06)×10⁹ /L]与NS组[(0.46±0.07)×10⁹ /L]比较，差异有显著性(P<0.05)。结论 该抗IL-5单抗具有下调外周血和气道EOS功能。

Preliminaryobservation of immunotherapy on asthmatic guinea pig

Shi Jieran, Sun Bin, ZhanXingli. Respiratory Department, Xijing Hospital, Xi ′ an, 710032

【 Abstract 】 Objective To observe the effect of theanti-IL-5 monoclonal antibody(McAb) on eosinophils of the guinea pigs with asthma. Method Sensitized guinea pigs by OVAwere divided into two groups randomly, which were treated with the McAb and normal saline(NS), respectively. Difference of peripheral blood eosinophil count (PBEC) among basal 、 pre-treatment and post-treatment,and the difference of bronchoalveolar lavage fluid eosinophil count (BALFEC) between twogroups above were compared. Result ThePBEC after treatment with the McAb [(1.63±0.26)×10⁹ /L] was lower thanthat before treatment [(1.76±0.27)×10⁹ /L] (P<0.05) and the BALFEC oftreatment group with the McAb was lower than that with NS [(0.41±0.06)×10⁹ /Lvs. (0.46±0.07)×10⁹ /L] (P<0.05). Conclusion The anti-IL-5 McAb has the ability to reduce the PBEC andthe BALFEC.

     【 Key words 】 Interleukins Monoclonal antibodies Asthma Animals, laboratory

哮喘是一种慢性气道炎症 ......

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