关键词:牙龈卟啉单胞菌;细胞外膜泡;多克隆抗体
摘 要 分别制备牙龈卟啉单胞菌47A-1及其膜泡的多克隆抗体,并用间接免疫荧光法对67个临床标本进行检测。结果经统计学检验,两种抗体显著相关,P<0.01,符合率为82.1%;膜泡抗体的检出率高于菌体抗体,P<0.05;膜泡抗体相对于菌体抗体的敏感性为93.5%。膜泡抗体与牙龈卟啉单胞菌以外的其他口腔细菌无交叉反应,说明膜泡抗体的特异性较强。
COMPARISON OF THE TWOKINDS OF POLYCLONAL
ANTIBODY OF PORPHYROMONAS GINGIVALIS
Fan Yaxian,Yang Shenghui.
Beijing Stomatological Hospital Capital University of Medicine,100050
Abstract In the present study,we preparedthe polyclonal antibody of P.g and the polyclonal antibody of ECV,and compared the twoantibodies using indirect immunofluorescence assay to 67 clinical samples.The two kinds ofantibody are significant correlated(p< 0.01), the accuracy is 82.1%.The positive rate of ECV antibody ishigher than that of P.g antibody (p< 0.05).The sensitivity of ECV antibody compared to P.g antibody is93.5%.The specificity of ECV antibody is very well because it does not cross-react withother oral strains.
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