许伟群 朱 苹 苏东辉 黄爱明 刘梁英 福建医科大学微生物学教研室(福州，350004) 中国人兽共患病杂志 1999 0 15 1


    关键词：脂多糖；抗体；ELISA；多粘菌素 期刊 zgrsghbzz 0 实验研究 fur -->


    

摘 要 由于细菌脂多糖(LPS)水溶性差，用传统的ELISA检测抗-LPS抗体抗原用量大，敏感性不理想。我们用能和LPS结合的多粘菌素B(PMB)预先包被微量反应板，可使随后加入的LPS(光滑型和粗糙型)包被效率增加，检测抗-LPS抗体(单克隆抗体和动物免疫血清)的敏感性显著增加(最高达128倍)。这一新方法对内毒素领域的研究和临床非常有用。

INCREASE OF THE SENSITIVITYOF ELISA FOR ANTI-LPS ANTIBODIES

    BY PRECOATING THE MICROTITER PLATES WITH POLYMYXIN B

Xu Weiqun Zhu Ping Su DonghuiHuang Aiming Liu Liangying

    (Department of Microbiology,Fujian Medical University,Fuzhou 350004)

ABSTRACT Conventional ELISA for anti-lipopolysaccharide(LPS)antibodies is not ideally sensitive because of the poorsolubility of bacterial LPS.A new ELISA by precoating the microtiter plates with ploymyxinB was developed,a cyclic antibiotic that is able to bind with LPS,to improve the coatingefficiency of LPS.In comparison with the conventional ELISA /in which LPS was directlycoated onto the plates,the new method detected much higher(128-fold as the highest)anti-LPS titers in murine antisera and monoclonal antibody raised against Re-LPS.

     ......

您现在查看是摘要页，全文长 14765 字符。