王军 张为国 虞爱华 王全立 刘耀清 马立人 王军 张为国 虞爱华 解放军302医院(北京，100039)；王全立 刘耀清 马立人 军事医学科学院二所 中国人兽共患病杂志 1999 0 15 1


    关键词：沙门氏菌肠炎；快速诊断；聚合酶链反应；序列分析 期刊 zgrsghbzz 0 实验研究 fur -->


    

摘 要 对沙门氏菌肠炎快速诊断，采用聚合酶链反应和DNA序列分析，以沙门氏菌保守序列——侵袭基因A作为靶序列，检测23株沙门氏菌均扩增出284bp特异的DNA片段，提示存在侵袭基因A，20株非沙门氏菌扩增阴性。检测84份粪标本，13份阳性，其中仅8份大便培养阳性。DNA序列分析证实粪标本扩增出的284bpDNA片段与侵袭基因A序列一致。结果表明聚合酶链反应用于沙门氏菌肠炎诊断，具有简便、快速、敏感等优点，适于临床快速诊断。

PRIMARY APPLICATION OF PCRTO RAPID

    DIAGNOSIS SALMONELLA ENTEROCOLITIS

Wang Jun Zhang Weigou Yu AihuaWang Qiuanli Liu Qioqing Ma Liren

(Institute of infectiousdiseases,Beijing,Fengtai Road 26,Beijing,10039)

ABSTRACT The methodof polymerase chain reaction(PCR)and DNA sequence analysis were used to detect salmonellaenteroclitis quickly.As the target DNA sequence the invA gene of 284bp DNA segments wereamplified in all 23 strains of salmonella by PCR and no any segments in 20 strains of nonsalmonella strains.84 feacil samples were amplified directily by PCR and 13 of them werepositive and 8 of them were positive in culture.It was confirmed that DNA sequences of284bp segments were consistent with the one of the invA gene.The results showed that PCRfor salmonella is simple,quick and sensitive,which is suitabl to apply in clinic quickdiagnosis of salmonella enterocolitis.

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