大鼠肝癌细胞 HSV-tk/GCV 自杀基因系统的构建及其旁观者效应
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谭宇蕙, 吴映雅, 杜标炎, 胡岳山, 赵 鹏,
HSV1-tk,GCV自杀基因系统;肝癌;大鼠;旁观者效应,大鼠肝癌细胞HSV-tk,GCV自杀基因系统的构建及其旁观者效应,谭宇蕙,吴映雅,胡岳山,,杜标炎,赵鹏,,李杰芬,,谭宇蕙,,通讯作者:
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谭宇蕙,吴映雅,胡岳山,广州中医药大学生化教研室 广东省广州市 510405
杜标炎,赵鹏,广州中医药大学病理教研室 广东省广州市 510405
李杰芬,广州中医药大学中医基础实验中心 广东省广州市 510405
谭宇蕙,女,1966-04-22生,广东省恩平市人,汉族,2002年广州中医药大学中西医结合专业博士,副教授,生化教研室主任,主要从事中西医结合分子肿瘤学研究.
国家自然科学基金资助项目,No. 30171201
广州市科技计划项目,No. 2002J1-C7041
通讯作者:杜标炎,510405,广州市机场路12号,广州中医药大学病理教研室. Tyuhui66@yahoo.com.cn
电话:020-36585475
收稿日期:2005-06-28 接受日期:2005-07-16
Construction of HSV-tk/GCV suicide gene system for hepatocarcinoma cells and its bystander effect in rats
Yu-Hui Tan, Ying-Ya Wu, Biao-Yan Du, Yue-Shan Hu, Peng Zhao, Jie-Fen Li
Yu-Hui Tan, Ying-Ya Wu, Yue-Shan Hu, Department of Biochemistry, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Biao-Yan Du, Peng Zhao, Department of Pathology, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Jie-Fen Li, Basic Experiment Center of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Supported by National Science Foundation of China, No. 30171201; and the Fund from Guangzhou Science and Technology Project, No.2002J1-C7041
Correspondence to: Biao-Yan Du, Department of Pathology, Guangzhou University of Traditional Chinese Medicine, 12 Jichang Road, Guangzhou 510405, Guangdong Province, China. Tyuhui66@Yahoo.com.cn
Received: 2005-06-28 Accepted: 2005-07-16
Abstract
AIM: To construct the therapeutic system of HSV-tk/GCV suicide gene for hepatocarcinoma cell line, and to establish a cell model and method to examine the effect of HSV-tk/GCV system and its bystander effect.
METHODS: CBRH7919 cells was infected by the active retrovirus particle excreted by the packaging cell line PT67/tk, and then a high anti-G418 clone named CBRH7919/tk was obtained by G418 screening for 3 wk ......
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