http://www.100md.com 唐印华, 田永刚, 刘铁夫, 徐洪雨, 庄丽维,
唐印华，刘铁夫，徐洪雨，庄丽维，梁桃，姜爱民，哈尔滨医科大学第一临床医学院消化内科 黑龙江省哈尔滨市 150001
田永刚，哈尔滨医科大学第四临床医学院普通外科 黑龙江省哈尔滨市 150001
Inhibitory effect of arsenic trioxide on proliferation of hepatoma cells in mice
Yin-Hua Tang, Yong-Gang Tian, Tie-Fu Liu, Hong-Yu Xu, Li-Wei Zhuang, Tao Liang, Ai-Min Jiang
Yin-Hua Tang, Tie-Fu Liu, Hong-Yu Xu, Li-Wei Zhuang, Tao Liang, Ai-Min Jiang, Department of Gastroenterology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Yong-Gang Tian, Department of General Surgery, the Forth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Supported by the 9th Five-Year Plan of Science and Technology of Heilongjiang Province, No. G98L19-1, and the Directive Project of Ministry of Health, No. 98-2-269
Correspondence to: Dr. Yin-Hua Tang, Department of Gastroenterology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China. email@example.com
Received: 2005-06-30 Accepted: 2005-07-15
AIM: To explore the arsenic trioxide(As2O3)-induced inhibition of the proliferation of mouse hepatoma cells in vivo.
METHODS: The mice bearing H22 solid and ascetic hepatoma cells were treated with different concentrations of arsenic trioxide. The growth of the solid tumor and the survival of the ascetic tumor-bearing mice were observed. The expression of proliferating cell nuclear antigen (PCNA) and cyclinD1 of the tumor cells were examined by immunohistochemical methods. The changes of cell cycle were measured by flow cytometry. The ultra-structural changes of the cells were observed under electron microscope.
RESULTS: Both high and low concentration of As2O3 inhibited the growth of the solid tumor and prolonged the survival time of ascetic-tumor-bearing mice, and the inhibitory rates were 39.1% and 45.7%, respectively. The life prolonging rates were 57.2% and 97.7%, respectively. The positive rates of PCNA and cyclinD1 expression in the As2O3-treated groups were significantly decreased from 57.9±6.6% to 44.0±5.0% (P <0.01), and from 49.2±9.3% to 37.6±6.3% (P <0.01), respectively. Flow cytometry showed that the percentage of G0/G1 phase cells was decreased and the percentage of G2/M phase cells was increased. Electron microscopy showed the typical characteristics of cell apoptosis ......