益气活血软坚解毒含药血清诱导人肝癌细胞系 Bel-7402 细胞的凋亡
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李东涛，孙桂芝，裴迎霞，祁鑫，李杰，林洪生
益气活血软坚解毒;肝癌;Bel-7402细胞,益气活血软坚解毒含药血清诱导人肝癌细胞系Bel-7402细胞的凋亡,李东涛，,孙桂芝，裴迎霞，祁鑫，李杰，林洪生，,李东涛,通讯作者：,电话：,收稿日期
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李东涛，第二军医大学附属长海医院中医科 上海市 200433
孙桂芝，裴迎霞，祁鑫，李杰，林洪生，中国中医研究院广安门医院肿瘤科 北京市 100053
李东涛, 1966-06-12生, 山东省汶上县人, 汉族, 2003年中国中医研究院博士, 主治医师, 现在第二军医大学从事博士后工作, 主要研究方向中西医结合治疗肝癌的基础与临床, 中医核心理论及虚弱体质研究工作.
通讯作者：孙桂芝, 100053, 北京市宣武区北线阁5号, 中国中医研究院广安门医院肿瘤科. whongzhong@263.net.
电话：010-88001630
收稿日期：2004-12-31 接受日期：2005-01-08
Growth inhibitory and apoptosis inducing effect on hepatocellular carcinoma Bel-7402 cell line by serum from rabbit fed with Yiqi Huoxue Ruanjian Jiedu decoction
Dong-Tao Li, Gui-Zhi Sun, Ying-Xia Pei, Xin Qi, Jie Li, Hong-Sheng Ling
Dong-Tao Li, Department of Traditional Chinese Medicine, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China
Gui Zhi-Sun, Ying-Xia Pei, Xin Qi, Jie Li, Hong-Sheng Ling, Guananmen Hospital, China Institute of Traditional Chinese Medicine, Beijing 100053, China
Correspondence to: Gui Zhi-Sun, Guanganmen Hospital, China Institute of Traditional Chinese Medicine, 5 Beixiange, Xuanwu District, Beijing 100053, China. whongzhong@263.net
Received: 2004-12-31 Accepted: 2005-01-08
Abstract
AIM: To investigate the growth inhibitory and apoptosis inducing effects on hepatocellular carcinoma Bel-7402 cell line by serum from rabbit fed with Yiqi Huoxue Ruanjian Jiedu decoction(YHRJ decoction).
METHODS: Serum was collected from New Zealand rabbits fed with YHRJ decoction, and used for Bel-7402 cell culture. Cell growth inhibition was assayed by MTT method. Invert, fluorescence, and laser microscopes were applied to examine cell morphology. Cell cycle and apoptosis were detected by annexinV and propidium iodide (PI) double staining and flow cytometry.
RESULTS: Serum from YHRJ-fed rabbits suppressed Bel-7402 cell growth. The inhibitory rate was 49.1%, when 20% serum was added to the culture. Typical apoptosis of morphological changes were also observed in YHRJ serum treated cells ......
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