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解偶联蛋白-2在大鼠非酒精性脂肪肝中的表达
http://www.100md.com 顾小红,张云东,冯爱娟
非酒精性脂肪;解偶联蛋白-2;免疫组化;Westernblot,顾小红,张云东,冯爱娟,顾小红,通讯作者:,Expressionofuncoupl
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     顾小红, 中国人民解放军第三军医大学大坪医院野战外科研究所消化科 重庆市 400042

    张云东, 中国人民解放军第三军医大学大坪医院野战外科研究所神经外科 重庆市400042

    冯爱娟, 北京军区白求恩国际和平医院干部一科 河北省石家庄市050082

    顾小红, 女,1968-06-01生,四川省绵阳盐亭县人,汉族,2004年第三军医大学在读博士,现任副主任医师,副教授,发表论文30多篇,参编专著7部,主要从事肝炎发病机理研究.

    重庆市科学基金应用基础研究项目,No.8108

    通讯作者: 顾小红,400042,重庆市,中国人民解放军第三军医大学大坪医院野战外科研究所消化科.zhanggxh68@sina.com

    收稿日期: 2005-08-01 接受日期: 2005-08-03

    Expression of uncoupling protein 2 in nonalcoholic fatty liver of rats

    Xiao-Hong Gu, Yun-Dong Zhang, Ai-Juan Feng

    Xiao-Hong Gu, Yun-Dong Zhang, Department of Gastroenterology,Daping Hospital of the Third Military Medical University, Chongqing400042, China

    Yun-Dong Zhang, Department of Neurological Surgery, DapingHospital of the Third Military Medical University, Chongqing 400042,China

    Ai-Juan Feng, the First Department of Cadre, BaiqiuenInternational Peace Hospital of Beijing Military Compound, Shijiazhuang050082, Hebei Province, China

    Supported by the Science Foundation of Chongqing for BasicApplicative Project, No.8108

    Correspondence to: Xiao-Hong Gu, Department of Gastroenterology,Daping Hospital of the Third Military Medical University, Chongqing400042, China. zhanggxh68@sina.com

    Received: 2005-08-01 Accepted: 2005-08-03

    Abstract

    AIM: To investigate the role of uncoupling protein-2 (UCP2) in thenonalcoholic fatty liver(NAFL) of rats.

    

    METHODS:
Sixty-four Wistar rats were randomly divided into twogroups: the control group and high-fat diet induced-fatty liver group.The expression of UCP2 antigen in the hepatocytes was examined byimmunohistochemistry and Western blot. Meanwhile, the levels of serumtriglyceride (TG), free fatty acid (FAA), and alanine aminotransferase(ALT)were measured by biochemical method.

    RESULTS:
The expression of UCP2 antigen and the number of UCP2positive cell were progressively increased at 2, 4, 8 and 12 wk, and thelevels of serum TG(0.78 ± 0.05, 0.85 ± 0.10, 1.16 ± 0.10, 1.39 ±0.07 mmol/L), FAA (371.3 ± 13.7, 439.2 ± 14.1, 486.3 ± 13.6, 636.7 ±20.3 μmol/L), and ALT (630.1 ± 41.7, 713.5 ± 75.0, 925.2 ± 105.0,1090.2 ± 65.0 nkat/L) in the fatty liver rats were increased than thosein the normal controls. Significant increase appeared at 8 and 12 wk (P<0.05 or P <0.01). Typical features of denaturation wereobserved in each animal.

    CONCLUSION:
The increase of UCP2 expression in NAFL is closelyassociated with the severity of liver inflammation and damages. UCP2 mayplay an important role in the progression of rat NAFL.

    Key Words: Nonalcoholic fatty liver; Uncoupling protein-2;Immunohistochemistry; Western blot

    Gu XH, Zhang YD, Feng AJ. Expression of uncoupling protein 2 innonalcoholic fatty liver of rats. Shijie Huaren Xiaohua Zazhi2005;13(19):2310-2313

    摘要

    目的探讨解偶联蛋白-2(uncoupling protein-2, UCP2) 在大鼠非酒精性脂肪肝发生中的作用 ......

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