超声辅助VEGF165转染骨髓间充质干细胞的实验研究
超声,,血管内皮生长因子；间充质干细胞；绿色荧光蛋白；转染,1材料与方法,2结果,3讨论,参考文献：
摘要：目的：探讨骨髓间充质干细胞定向分化血管内皮细胞的可行性研究。方法：采用超声辅助电穿法，将VEGF165基因的质粒PEGFP-C1转染至骨髓间充质干细胞，转染后于荧光显微镜下观察绿色荧光蛋白(GFP)表达情况。结果：超声辅助转染5h后可见细胞内有GFP表达，10h后可达高峰。结论：VEGF基因转染骨髓间充质干细胞后，骨髓间充质干细胞内有GFP表达，提示细胞转染成功，骨髓间充质干细胞定向分化为血管内皮细胞具有可行性。关键词：血管内皮生长因子；间充质干细胞；绿色荧光蛋白；转染
Experimental Study on VEGF165 Transfection of Medulla MSCs by Ultrasound
WANG Tao，JI Jun，et al
(Shenzhen People’s Hospital，Guangdong Shenzhen 518000，China)
Abstract： Objective:To study the feasibility of medulla MSCs differentiation directionally to endothelial cells. Method:Observe the expression of green fluorescence protein(GFP) in medulla MSCs after transfection of VEGF165 vector,PEGFP-C1 by ultrasound under the fluorescence microscope.Result:GFP expression was seen 5 hours after transfection,and reached the peak 10 hours later.Conclusion: It is possible that medulla MSCs could be differentiate directionally to endothelial cells after the transfection of VEGF vector.
Key words:VEGF；Mesenchymal stemcells；GFP；Transfection
缺血性心脏病是冠状动脉狭窄或闭塞导致的心肌缺血从而影响心脏功能的一类疾病 ......
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