Troglitazone对小鼠Ⅰ型葡萄糖载体表达的影响
Troglitazone,,Troglitazone；小鼠Ⅰ型葡萄糖载体；mRNA表达,1材料和方法,2结果,3讨论,参考文献：
摘要：目的：探讨Troglitazone对小鼠I型葡萄糖载体(mGLUT1)表达的影响。方法：采用分子克隆技术，将过氧化物酶体增殖物受体γ(PPARγ)和维甲酸类受体X(RXRα)及 mGLUT1 cDNA分别克隆到表达载体pCMX和pGL3b上。PPARγ与RXRα及mGLUT1克隆载体转染NIH 3T3细胞，处理或不处理Troglitazone，应用荧光素酶活性测定法及RNA印迹等方法测定Troglitazone对mGLUT1重组体荧光素酶活性调节及对mRNA表达的影响。结果：Troglitazone可激活mGLUT1重组体荧光素酶的活性，并且可增加mGLUT1的mRNA表达水平。结论：Troglitazone可增强mGLUT1的表达，可能参与PPARγ对mGLUT1的调节过程。关键词：Troglitazone；小鼠Ⅰ型葡萄糖载体；mRNA表达
Effect of Troglitazone on Mouse Glucose Transporter I(mGLUT1) Expression
LI Tianzhu, LIU Mingzhu
(Medical College, Yanbian University， Yanji 133000, China)
Abstract:Objective:To understand the possibility of effect of troglitazone on mGLUT1 expression.Methods:PPARγ , RXRα and mGLUT1 cDNA clone into pCMX and pGL3b expression vector. The recombinant DNA transfected into NIH 3T3 cells, treated trogiltazone and confirm the mGLUT1 activity and mRNA expression by the trogiltazone through PPARγ activation with luciferase assay and Northern blot.Results:Troglitazone activates the mGLUT1 activity and increase the mGLUT1 mRNA expression through PPARγ activation. Conclusion:Troglitazone binds to PPARγ and activates mGLUT1 expression. ......
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