胃肿瘤_____胃肿瘤·DNA，线粒体·基因突变·多态现象

胃癌线粒体DNA Dloop区突变的检测和分析高壮雷

http://www.100md.com 《中国普通现代外科进展》 2005年第5期

胃肿瘤,,胃肿瘤·DNA，线粒体·基因突变·多态现象,胃癌线粒体DNADloop区突变的检测和分析高壮雷,1研究对象与方法,2结果,3讨论,参考文献

     【摘要】目的：探讨线粒体DNA(mtDNA)的D环(Dloop)区16104191bp序列在胃癌中的突变情况。方法：选择12例胃癌病人的肿瘤组织及其邻近的正常胃粘膜，用PCR方法将mtDNA Dloop区同时扩增测序。结果：在12例胃癌组织中有6例存在mtDNA Dloop区16104191bp片段的突变，突变率为50%，总计有16个突变位点。12例中共发现28个多态性变化，其中有3个基因库未记载的新的多态性变化。结论：mtDNA Dloop区是一个具有高度多态性和突变性的区域，该区在胃癌病人中是mtDNA突变的热区。

    【关键词】胃肿瘤·DNA，线粒体·基因突变·多态现象

    Mutations in the Dloop region of mitochondrial DNA in gastric cancer

    GAO Zhuanglei,ZHANG Zongli,LIU Shuping,WANG Wei,GAO Qianfu

    1Department of General Surgery,2Department of Neurology,Qilu Hospital of Shan

    dong University (Jinan 250012,China)

    3Experimental Center of Medical Molecular Biology,Shandong University(Jinan

    250012,China)

    【ABSTRACT】Objective:To investigate the variations of mitochondrial Dloop region in gastric cancers.Methods:The Dloop region of 12 gastric cancers together with the adjacent normal tissues were amplified by PCR and sequenced.Results: Among the 12 cases,16 mutations and 28 polymorphisms were identified with the mutations rate of 50%.There were 3 new polymophisms which were not reported previously in the GeneBank among the 28 polymorphisms.Conclusion:We think that the mitochondrial DNA Dloop region is a highly polymorphric and mutable region and the mutation rate is relatively high in patients with gastric cancer. ......

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