【摘要】 道地药材和非道地药材在形态、生药性状及化学成分等特征上具有高度相似性，因而传统的鉴别方法对道地药材的鉴别往往不易。本文首次将扩增片段长度多态性技术(AFLP，amplified fragment length polymorphism)应用到中药材道地性的鉴别中，绘制不同产地中药材的AFLP指纹图谱和遗传多样性，确定道地性产区药材的特异性差异带，从而区分道地药材。在此基础上，将AFLP标记转换成简单实用的SCAR(特征性片段扩增区域，sequence characterized amplified region)标记。将SCAR标记片段进行胶回收，PCR或克隆测序，然后根据序列特点设计特异性引物，通过简单的PCR，即可鉴别道地性产地中药材。在资源调查、鉴定研究的基础上，建立中药材道地性评价体系，为最终从分子生物学水平上建立中药材道地性的统一质量控制标准，提供简便、快速、准确的鉴别技术。

    【关键词】 中药材；道地性；AFLP；SCAR；鉴别

    Application of the AFLP combining with SCAR to identify the geoherbalism of traditional Chinese medicines

    HAO Ming-gan， LIU Zhong-quan.

    Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection， Department of Biology， Yancheng Teachers College， Yancheng， Jiangsu 224002，China

    【Abstract】 The characters from morphologic and pharmacognosy and chemic components are high similar beteen genuine and ungenuine crude drugs. It is very difficult to identify the geoherbalism of the crude drugs with traditional methods. In this paper， we first applied the technique of the amplified fragment length polymorphism (AFLP) to distinguish the geoherbalism of traditional Chinese medicines. Fingerprint maps of crude drugs from different regions were drawn ......