http://www.100md.com 吴小南, 陈 洁, 汪家梨, 林建银
吴小南, 陈洁, 汪家梨,福建医科大学公共卫生学院 福建省福州市 350004
林建银, 福建医科大学分子医学研究中心 福建省福州市 350004
吴小南, 男, 1963-07-28生, 福建省南安人, 汉族, 2004年福建医科大学病原生物学博士, 教授, 主要从事营养与保健资源开发研究.
通讯作者: 林建银, 350004, 福建省福州市交通路88号, 福建医科大学分子医学研究中心. email@example.com
电话: 0591-83569450 传真: 0591-83351345
收稿日期: 2005-08-29 接受日期: 2005-09-06
Modulatory role of lotus-seed milk fermented product on gastrointestinal motility and absorption in mice
Xiao-Nan Wu, Jie Chen, Jia-Li Wang, Jian-Yin Lin
Xiao-Nan Wu, Jie Chen, Jia-Li Wang, School of Public Health, Fujian Medical University, Fuzhou 35004, Fujian Province, China
Jian-Yin Lin, Research Center of Molecular Medicine, Fujian Medical University, Fuzhou 35004, Fujian Province, China
Supported by the Key Program Foundation of Ministry of Education of China, No.02074
Correspondence to: Dr. Jian-Yin Lin, Research Center of Molecular Medicine, Fujian Medical University, Fuzhou 35004, Fujian Province, China. firstname.lastname@example.org
Received: 2005-08-29 Accepted: 2005-09-06
AIM: To explore the modulatory role of lotus-seed milk fermented product (LMFP) on gastrointestinal motility and absorption function in mice.
METHODS: Mice were divided into groups by the ran-domized complete weight-block design and treated with distilled water, different drugs and different conc-entrations of LMFP accordingly. L-arginine was intrape-ritoneally injected into the mice to observe the state of gastric emptying, small intestinal propelling, and the nitric oxide (NO) concentration in gastric homogenate; Compound Diphenoxylate was used to establish the mouse model of constipation, and the time, weight, quantity and the water content of black feces were measured; Reserpine was subcutaneously injected into the mice to calculate the small intestinal propellant rate and the content of uric xylose.
RESULTS: The delay of gastric emptying was positive-ly correlated with the NO concentration (r = 0.475, P = 0.001). As compared with those in the l-arginine model group, the volumns of phenol red leaving in the stomach of 250, 500 mL/L LMFP groups and Dom-peridone group were significantly decreased (22.78±6.95%, 27.12±5.73%, 22.82±5.63% vs 34.76±9.15%, P <0.05) and the NO concentration of gastric homogenate in 250 mL/L LMFP group and Domperido-ne group were lower (26.13±4.95 µmol/g pro, 26.33±4.06 µmol/g pro vs 38.33±9.82 µmol/g pro, P <0.05). The volumns of phenol red leaving in the distal 3 small intestinal segments of 500 mL/L, 750 mL/L LMFP group and Domperidone group were markedly higher, and the volumns leaving in the distal 4 small intestinal segments in 750 mL/L LMFP group and Domperidone group were also notably increased (P <0.05), in comparison with those in the model group. The time of black feces excreted was reduced and the water content of the feces were significantly increased in 250, 500, 750 mL/L LMFP groups than those in the model group (133 ......