塞莱昔布对结肠癌细胞生长及肝转移瘤血管生长因子表达的影响
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张 超, 刘 伟, 安 杰, 刘占奎
结肠肿瘤;环氧合酶;塞莱昔布,张超,刘伟,刘占奎,安杰,通讯作者:,电话:,收稿日期:,接受日期:,Effectsofcelecoxibo
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张超, 刘伟, 刘占奎, 第三军医大学西南医院普外科 重庆市 400038
安杰, 白求恩国际和平医院病理科 河北省石家庄市 050082
通讯作者: 张超, 400038, 重庆市第三军医大学西南医院普通外科. zhangchao5642@163.com
电话: 023-68773121
收稿日期: 2005-08-29 接受日期: 2005-09-17
Effects of celecoxib on cell proliferation and expression of vascular endothelial growth factor in colonic cancer with hepatic metastasis
Chao Zhang, Wei Liu, Jie An, Zhan-Kui Liu
Chao Zhang, Wei Liu, Zhan-Kui Liu, Department of General Surgery, Southwest Hospital of the Third Military Medical University, Chongqing 400038, China
Jie An, Department of Pathology, Bethune International Peace Hospital, Shijiazhuang 050082, Hebei Province, China
Correspondence to: Chao Zhang, Department of General Surgery, Southwest Hospital of the Third Military Medical University, Chongqing 400038, China. zhangchao5642@163.com
Received: 2005-08-29 Accepted: 2005-09-17
Abstract
AIM: To explore the role of celecoxib in the proliferation and cycle distribution of colon cancer cells cultured in vitro as well as its effect on the expression of vascular endothelial growth factor (VEGF) in the animal model of colon cancer with hepatic metastasis.
METHODS: The human colon cancer cells HT-29 and HCT-116 were employed in the study. After treatment with celecoxib, the inhibitory effect of celecoxib on the proliferation of cancer cells was determined by MTT assay, and the cell cycle distribution was detected by flow cytometry. The tumor cells were inoculated in the nude mice, and the expression of VEGF in the model was detected by immunohistochemistry.
RESULTS: Celecoxib inhibited the proliferation of the tumor cells in a time- and dose-dependent manner. The inhibitory effect on HT-29 cells was stronger than that on HCT-116 cells (P <0.01). Celecoxib changed the cell cycle distribution of both cells, and decreased the proliferation index of cells obviously (P <0 ......
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