银杏叶类黄酮对人胃癌细胞 BGC823 体外的增殖抑制作用
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张 凤, 杨桂文, 张金凤, 安利国
银杏叶类黄酮;胃癌,银杏叶类黄酮对人胃癌细胞BGC823体外的增殖抑制作用,张凤,杨桂文,张金凤,安利国,通讯作者:,电话:,收稿日期:,接受日期:,InhibitoryeffectsofGinkg
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张凤, 杨桂文, 张金凤, 安利国, 山东师范大学生命科学学院动物抗性重点实验室 山东省济南市 250014
通讯作者: 安利国, 250014, 山东省济南市文化东路88号, 山东师范大学生命科学学院. anlg@sdnu.edu.cn
电话: 0531-86180143
收稿日期: 2005-06-28 接受日期: 2005-07-16
Inhibitory effects of Ginkgo biloba leaf flavonoids on proliferation of human gastric cancer cell line BGC823 in vitro
Feng Zhang, Gui-Wen Yang, Jin-Feng Zhang, Li-Guo An
Feng Zhang, Gui-Wen Yang, Jin-Feng Zhang, Li-Guo An, Key Laboratory of Animal Resistance, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong Province, China
Correspondence to: Li-Guo An, College of Life Sciences, Shandong Normal University, 88 Wenhua East Road, Jinan 250014, Shandong Province, China. anlg@sdnu.edu.cn
Received: 2005-06-28 Accepted: 2005-07-16
Abstract
AIM: To extract the flavonoids from Ginkgo biloba leaf, and to investigate its inhibitroy effects on the proliferation of human gastric cancer cell line BGC823 cultured in vitro.
METHODS: Ethanol (700 mL/L) was used to extract the flavonoids from the leaf of Ginkgo biloba. Three wavelength spectrophotometry was used to determine the content of flavonoids in the extracts. Human gastric cancer cells BGC823 cultured in vitro were treated with different concentrations of the flavonoids, and then the proliferation of the cells was detected by MTT assay and flow cytometry.
RESULTS: The content of flavonoids in the extracts was 140 mg/g. The flavonoids from Ginkgo biloba leaf inhibited the proliferation of BGC823 cells in a dose-dependent manner. The rate of cells in S phase was notably increased as compared with that in the controls (42.17±0.50%vs 32.13±0.45%, P = 0.001), and the apoptotic rate of the cells was also increased (4.10±0.03% vs 2.21±0.01%, P = 0.002).
CONCLUSION: Ginkgo biloba leaf flavonoids can inhibit the proliferation of human gastric cancer cell line BGC823 by affecting the cycle the cells ......
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