【摘要】 目的 使用99mTc示踪初步研究注射用NGF在家兔体内药代动力学。方法 将NGF用99mTc标记，利用同位素示踪法和TCA沉淀法测得NGF含量与放射性计数的线性关系，并由静脉注射至家兔体内，通过上述检测方法得到不同时段血浆中微量NGF浓度，使用3P87软件分析药代动力学参数并初步分析组织分布。结果 NGF静脉注射后的家兔体内代谢符合二隔室分布模型；按剂量40μg/kg静注后，测得T1/2α=0.077h、T1/2β=0.88h、AUC=98.6514ng·h·ml-1。结论 99mTc具有较好的示踪效果，适用于体内药代动力学研究。

    【关键词】 99mTc-NGF；药代动力学；TCA沉淀法

    Pharmacokinetic research of nerve growth factor marked with99mTc in rabbit

    FU Chang-wen，LU Tian-run， CAO Xiao-jian，et al.

    Department of Surgery， The First and Second Affiliated Hospital of Nanjing Medical University， NanJing 210029， China

    【Abstract】 Objective To study the pharmacokinetics of NGF in rabbit by isotopic tracing. Methods NGF was labeled with99mTc，and its γ-ray counts was detected by isotopic tracing and trichloroacetic acid ( TCA) precipitation method. From cpm of γ-ray and NGF content of samples， the standard curve was drawn and it was nearly a line.99mTc-NGF was injected into rabbit(n=30) body from vena auris . Samples of rabbit blood were collected in different time (0.00833 ......