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从养殖蛙及池水中检出51株O1群霍乱弧菌非流行株报告
http://www.100md.com 《中国热带医学杂志》 2005年第2期
群霍乱弧菌,,群霍乱弧菌;非流行株;噬菌体生物分型,1材料与方法,2结果,3讨论,参考文献:
     摘要:目的 通过对人工养殖蛙及其池水中O1群霍乱弧菌检测,了解霍乱弧菌在其中的存活及菌型分布情况,为制定防治措施提供依据。 方法 采集人工养殖蛙及养蛙池水进行霍乱弧菌分离培养,血清学诊断符合O1群霍乱弧菌者,按国标法进行系统菌株鉴定。 结果 从624份标本中检出51株O1群E1-Tor霍乱弧菌,总检出率为8.17%,其中稻叶型42株,占82.4%,小川型9株,占17.6%;噬菌体—生物分型:5K29株,占56.9%,其次为9L8株、5L5株、13K5株、9K2株、12K2株。 结论 检出51株O1群 E1-Tor霍乱弧菌均属非流行株。池水检出率高于蛙的检出率。总检出率较高,提示在今后霍乱监测中,这些环境应作为重点之一。

    关键词:群霍乱弧菌;非流行株;噬菌体生物分型

    中图分类号:R378.3 文献标识码:B 文章编号:1009-9727(2005)02-375-02

    Report of 51 non-epidemic strains of Vibrio chloerae O1 group detected from frogs and cultivating pool HUANG Chang-he, Wu Jie, WANG Jia-hao, et al (Hainan Provincial Center for Disease Control and Prevention, Haikou 570203, Hainan, P RChina)

    Abstract Objective To understand the survival and distribution of types of V cholerae to provide basis for drawing out control and prevention measures through testing V cholerae O1 from farmed frogs and pool water Methods Collecting samples of farmed frogs and the pool water to isolate V cholerae If the strains were V cholerae O1 through diagnosis of serology they would be identified systematically according to the national standard Results 51 strains of V cholerae of El-Tor biotype were tested from 624 samples The rate was 817% In that 42 strains were Inaba type (824%) 9 strains were Ogawa type (176%) The results of bacteriophage-biotype were that 569% of them was 5K (29 strains) and others were 9L (8 strains), 5L (5 strains), 13K (5 strains), 9K (2 strains) and 12K (2 strains) Conclusions All the 51 strains of V cholerae of El-Tot biotype were non-epidemic strains The rate of samples from pool water was higher than that of frogs In general the rate was rather high It prompted that the aquatic farm was one of the focal points for surveillance of V cholerae ......

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