纳米细菌的免疫电镜检测
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王利民, 沈文律, 周 珍, 张士莲
纳米细菌;免疫电镜;细菌被膜,王利民,沈文律,周珍,张士莲,通讯作者:,Detectionofnanobacteriabyimmuneelectronicmicrosc
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王利民, 唐山工人医院普通外科 河北省唐山市 063000
沈文律, 汕头大学医学院附属第二医院 广东省汕头市 515041
周珍, 华北煤炭医学院 河北省唐山市 063000
张士莲, 开滦矿务局医院 河北省唐山市 063000
通讯作者: 王利民, 063000, 河北省唐山市文化路27号, 唐山工人医院普通外科. g_lmwang@sina.com.cn
电话: 0315-3722208 传真: 0315-3722104
收稿日期: 2005-08-06 接受日期: 2005-08-26
Detection of nanobacteria by immune electronic microscopy
Li-Min Wang, Wen-Lv Shen, Zhen Zhou, Shi-Lian Zhang
Li-Min Wang, Department of General Surgery, Tangshan Worker’s Hospital, Tangshan 063000, Hebei Province, China
Wen-Lv Shen, the Second Affiliated Hospital, Medical College of Shantou University, Shantou 515043, Guangdong Province, China
Zhen Zhou, North China Coal Medical College, Tangshan 063000, Hebei Province, China
Shi-Lian Zhang, Hospital of Kailuan Mining Bureau, Tangshan 063000, Hebei Province, China
Correspondence to: Li-Min Wang, Department of General Surgery, Tangshan Worker’s Hospital, 27 Wenhua Road, Tangshan 063000, Hebei Province, China. g_lmwang@sina.com.cn
Received: 2005-08-06 Accepted: 2005-08-26
Abstract
AIM: To introduce an effective method of immunogold electron microscopy for the detection of nanobacteria.
METHODS: Nanobacteria were fixed in the 25 g/L glut-araldehyde, and then were embedded in resin. After they were cut into ultrathin slices by diamond knife, an indirect immunogold electronic microscopy staining was performed on the nanobacteria using the specific antibody of nanobacteria.
RESULTS: The nanobacteria were ball- or stick-like with a length of 80-350 nm under electron microscope. Nanobacteria were combined with the specific antibo-dy. The adhesion of the immune colloidal gold granule was observed under the electronic microscopy ......
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