推拉动作_____推拉动作；海马；神经元；损伤

模拟高强度推拉动作对大鼠海马神经元损伤的实验研究

http://www.100md.com 《吉林医药医学学报》 2005年第1期

推拉动作,,推拉动作；海马；神经元；损伤,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的探讨大鼠经高强度模拟推拉动作暴露后，海马不同区域神经元的损伤程度和时相变化规律。方法健康雄性Wistar大鼠20只，随机分为正常对照组5只，实验组(8Gz → +8Gz组)15只。正常对照组不做任何处理，实验组大鼠分别在暴露后30 min、6 h、24 h取材，灌流固定；石蜡切片，HE染色，光镜观察；超薄切片，透射电镜观察。结果±8Gz暴露后6 h和24 h海马各区可见到一定数量形态发生改变的神经元，以CA1区损伤最重。结论高强度推拉动作可造成海马神经元的损伤。

    关键词：推拉动作；海马；神经元；损伤

    The experimental study on the injury of hippocampal neurons of rats caused by simulated highintensity pushpull maneuver

    GUO Suhong, XU Ye, XU Bin, LIU Shifu，LI Yan, CHEN Moran

    (Jilin Medical College,Jilin City,Jilin Province,132013,China)

    Abstract:ObjectiveTo explore the injury of hippocampus neurons in different areas and time phase change in rats after expose to stimulated high intensity pushpull maneuver.MethodsTewenty healthy male Wistar rats were randomly divided into the control group(5 rats) and experimental group(-8Gz → +8Gz) (15rats). Nothing was done for the rats in the control groups while the rats in the experimental groups were perfused and fixed and cut respectively at 0.5hour,6hour and 24hour after exposure for HE staining for observing of light microscope and ultrathin sectioning for analysis of ultrastructure.ResultsThere are some neurons with morphological change in different areas of hippocampus after 6hour and 24hour exposure to ±8Gz and the injury was the most severe in CA1 area. ConclusionThe intensity high pushpull maneuver may cause more injury of hippocampus tissues. ......

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